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From the same journal

Quantifying Secondary Structure Changes in Calmodulin Using 2D-IR Spectroscopy

Research output: Contribution to journalArticle

Author(s)

  • Lucy Minnes
  • Daniel J Shaw
  • Benjamin P Cossins
  • Paul M Donaldson
  • Gregory M Greetham
  • Michael Towrie
  • Anthony W Parker
  • Matthew J Baker
  • Alistair J Henry
  • Richard J Taylor
  • Neil T Hunt

Department/unit(s)

Publication details

JournalAnalytical Chemistry
DateAccepted/In press - 18 Sep 2017
DateE-pub ahead of print - 29 Sep 2017
DatePublished (current) - 17 Oct 2017
Issue number20
Volume89
Number of pages9
Pages (from-to)10898-10906
Early online date29/09/17
Original languageEnglish

Abstract

(Graph Presented) Revealing the details of biomolecular processes in solution needs tools that can monitor structural dynamics over a range of time and length scales. We assess the ability of 2D-IR spectroscopy in combination with multivariate data analysis to quantify changes in secondary structure of the multifunctional calcium-binding messenger protein Calmodulin (CaM) as a function of temperature and Ca 2+ concentration. Our approach produced quantitative agreement with circular dichroism (CD) spectroscopy in detecting the domain melting transitions of Ca 2+ -free (apo) CaM (reduction in α-helix structure by 13% (CD) and 15% (2D)). 2D-IR also allows accurate differentiation between melting transitions and generic heating effects observed in the more thermally stable Ca 2+ -bound (holo) CaM. The functionally relevant random-coil-α-helix transition associated with Ca 2+ uptake that involves just 7-8 out of a total of 148 amino acid residues was clearly detected. Temperature-dependent Molecular Dynamics (MD) simulations show that apo-CaM exists in dynamic equilibrium with holo-like conformations, while Ca 2+ uptake reduces conformational flexibility. The ability to combine quantitative structural insight from 2D-IR with MD simulations thus offers a powerful approach for measuring subtle protein conformational changes in solution.

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