Quantifying single-cell secretion in real time using resonant hyperspectral imaging

José Juan-Colás; Ian S Hitchcock; Mark Coles; Steven Johnson; Thomas F Krauss

doi:10.1073/pnas.1814977115

Quantifying single-cell secretion in real time using resonant hyperspectral imaging

José Juan-Colás^*, Ian S Hitchcock, Mark Coles, Steven Johnson, Thomas F Krauss

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

Original language	English
Pages (from-to)	13204-13209
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	115
Issue number	52
Early online date	10 Dec 2018
DOIs	https://doi.org/10.1073/pnas.1814977115
Publication status	Published - 26 Dec 2018

Bibliographical note

Keywords

Label-free
Photonic biosensing
Photonic crystal
Single-cell analysis

Access to Document

10.1073/pnas.1814977115

Manuscript
© 2018, The Author(s).
Accepted author manuscript, 67.9 KBLicence: CC BY

Cite this

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abstract = "Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.",

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AU - Juan-Colás, José

AU - Hitchcock, Ian S

AU - Coles, Mark

AU - Johnson, Steven

AU - Krauss, Thomas F

PY - 2018/12/26

Y1 - 2018/12/26

N2 - Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

AB - Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

KW - Label-free

KW - Photonic biosensing

KW - Photonic crystal

KW - Single-cell analysis

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U2 - 10.1073/pnas.1814977115

DO - 10.1073/pnas.1814977115

M3 - Article

C2 - 30530663

SN - 0027-8424

VL - 115

SP - 13204

EP - 13209

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 52

ER -

Quantifying single-cell secretion in real time using resonant hyperspectral imaging

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Thomas Fraser Krauss

Resonant and shaped photonics for understanding the physical and biomedical world

Biophysics of Infection and Immunity: from Molecules to Cells to Tissues