Projects per year
Abstract
Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.
Original language | English |
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Pages (from-to) | 13204-13209 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 115 |
Issue number | 52 |
Early online date | 10 Dec 2018 |
DOIs | |
Publication status | Published - 26 Dec 2018 |
Bibliographical note
© 2018, The Author(s).Keywords
- Label-free
- Photonic biosensing
- Photonic crystal
- Single-cell analysis
Profiles
Projects
- 2 Finished
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Resonant and shaped photonics for understanding the physical and biomedical world
Krauss, T. F. (Principal investigator) & Johnson, S. D. (Co-investigator)
1/08/17 → 31/12/22
Project: Research project (funded) › Research
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Biophysics of Infection and Immunity: from Molecules to Cells to Tissues
Coles, M. C. (Principal investigator), Baumann, C. G. (Co-investigator), Bees, M. A. (Co-investigator), Johnson, S. D. (Co-investigator), Kaye, P. (Co-investigator), Krauss, T. F. (Co-investigator), Leake, M. C. (Co-investigator), O'Toole, P. J. (Co-investigator), Potts, J. R. (Co-investigator), Rot, A. (Co-investigator) & Van Der Woude, M. (Co-investigator)
1/02/16 → 28/02/19
Project: Research project (funded) › Research
Datasets
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Quantifying single cell secretion in real time using resonant hyperspectral imaging
Juan-Colas, J. (Creator), Hitchcock, I. S. (Creator), Coles, M. C. (Creator), Johnson, S. D. (Creator) & Krauss, T. F. (Creator), University of York, 2018
DOI: 10.15124/dffb9f91-cd1e-490e-8813-da88da18f16b
Dataset