Quantifying single-cell secretion in real time using resonant hyperspectral imaging

Research output: Contribution to journalArticlepeer-review


Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

Original languageEnglish
Pages (from-to)13204-13209
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number52
Early online date10 Dec 2018
Publication statusPublished - 26 Dec 2018

Bibliographical note

© 2018, The Author(s).


  • Label-free
  • Photonic biosensing
  • Photonic crystal
  • Single-cell analysis

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