Projects per year
Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.
|Number of pages||6|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Early online date||10 Dec 2018|
|Publication status||Published - 26 Dec 2018|
Bibliographical note© 2018, The Author(s).
- Photonic biosensing
- Photonic crystal
- Single-cell analysis
- 2 Finished
1/08/17 → 31/12/22
Project: Research project (funded) › Research