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From the same journal

Quantifying single-cell secretion in real time using resonant hyperspectral imaging

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Publication details

JournalProceedings of the National Academy of Sciences of the United States of America
DateAccepted/In press - 6 Nov 2018
DateE-pub ahead of print - 10 Dec 2018
DatePublished (current) - 26 Dec 2018
Issue number52
Number of pages6
Pages (from-to)13204-13209
Early online date10/12/18
Original languageEnglish


Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

Bibliographical note

© 2018, The Author(s).

    Research areas

  • Label-free, Photonic biosensing, Photonic crystal, Single-cell analysis

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