Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR

Guido Cordoni; Adele Williams; Andy Durham; Daniela Florio; Renato Giulio Zanoni; Roberto M La Ragione

doi:10.1016/j.rvsc.2015.08.008

Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR

Guido Cordoni, Adele Williams, Andy Durham, Daniela Florio, Renato Giulio Zanoni, Roberto M La Ragione

Language and Linguistic Science

Research output: Contribution to journal › Article › peer-review

Abstract

Strangles is one of the most common equine infectious diseases with serious health, welfare and socio-economic impact. However, the detection of Streptococcus equi subspecies equi can be challenging and persistently infected carriers are common. Furthermore, the use of classical microbiology can result in an underestimation of the prevalence of the disease. The difficulties associated with the slow diagnosis of Strangles can result in rapid spread of the disease. Therefore, rapid and economical diagnostic tests are urgently required. Here, two multiplex assays, were developed and validated for the detection of S. equi and S. equi subspecies zooepidemicus, the most common differential diagnosis. Using 59 S. equi and 59 S. zooepidemicus strains collected from various geographical areas, the PCR tests demonstrated a sensitivity of 95% and a specificity of 98%. Furthermore, the assay can be performed directly from clinical swabs. Thus, the assays designed here provide a rapid, reliable and economical solution for the diagnosis of Strangles.

Original language	English
Pages (from-to)	162-166
Number of pages	5
Journal	Research in Veterinary Science
Volume	102
DOIs	https://doi.org/10.1016/j.rvsc.2015.08.008
Publication status	Published - Oct 2015

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10.1016/j.rvsc.2015.08.008

Cite this

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title = "Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR",

abstract = "Strangles is one of the most common equine infectious diseases with serious health, welfare and socio-economic impact. However, the detection of Streptococcus equi subspecies equi can be challenging and persistently infected carriers are common. Furthermore, the use of classical microbiology can result in an underestimation of the prevalence of the disease. The difficulties associated with the slow diagnosis of Strangles can result in rapid spread of the disease. Therefore, rapid and economical diagnostic tests are urgently required. Here, two multiplex assays, were developed and validated for the detection of S. equi and S. equi subspecies zooepidemicus, the most common differential diagnosis. Using 59 S. equi and 59 S. zooepidemicus strains collected from various geographical areas, the PCR tests demonstrated a sensitivity of 95% and a specificity of 98%. Furthermore, the assay can be performed directly from clinical swabs. Thus, the assays designed here provide a rapid, reliable and economical solution for the diagnosis of Strangles.",

author = "Guido Cordoni and Adele Williams and Andy Durham and Daniela Florio and Zanoni, {Renato Giulio} and {La Ragione}, {Roberto M}",

year = "2015",

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doi = "10.1016/j.rvsc.2015.08.008",

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T1 - Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR

AU - Cordoni, Guido

AU - Williams, Adele

AU - Durham, Andy

AU - Florio, Daniela

AU - Zanoni, Renato Giulio

AU - La Ragione, Roberto M

PY - 2015/10

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AB - Strangles is one of the most common equine infectious diseases with serious health, welfare and socio-economic impact. However, the detection of Streptococcus equi subspecies equi can be challenging and persistently infected carriers are common. Furthermore, the use of classical microbiology can result in an underestimation of the prevalence of the disease. The difficulties associated with the slow diagnosis of Strangles can result in rapid spread of the disease. Therefore, rapid and economical diagnostic tests are urgently required. Here, two multiplex assays, were developed and validated for the detection of S. equi and S. equi subspecies zooepidemicus, the most common differential diagnosis. Using 59 S. equi and 59 S. zooepidemicus strains collected from various geographical areas, the PCR tests demonstrated a sensitivity of 95% and a specificity of 98%. Furthermore, the assay can be performed directly from clinical swabs. Thus, the assays designed here provide a rapid, reliable and economical solution for the diagnosis of Strangles.

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