Rapid Discrimination of Archaeal Tetraether Lipid Cores by Liquid Chromatography-Tandem Mass Spectrometry

TY - JOUR

T1 - Rapid Discrimination of Archaeal Tetraether Lipid Cores by Liquid Chromatography-Tandem Mass Spectrometry

AU - Knappy, Christopher S.

AU - Chong, James P. J.

AU - Keely, Brendan J.

PY - 2009/1

Y1 - 2009/1

N2 - Atmospheric pressure chemical ionization liquid chromatography-tandem mass spectrometry (APCI LC-MS/MS) of tetraether lipid cores of archaeal origin reveals distinct dissociation pathways for three classes of core lipid extracted from Methanobacter thermautotrophicus. Within these classes, two isobaric tetraether lipids, one a scarcely reported lipid constituent of M. thermautotrophicus and the other an artefact formed during extraction from cultured cells, were identified and distinguished via their MS2 spectra. APCI LC-MS/MS discriminates different tetraether core lipid types and isobaric species and reveals the mass of the constituent biphytanyl chains within the tetraether cores, albeit without full elucidation of their structures. Furthemore, the method allows direct estimation of the relative proportions of tetraether core lipids from chromatographic peak area measurement, allowing rapid profiling of these compounds in microbiological and environmental extracts. (J Am Soc Mass Spectrom 2009, 20, 51-59) (C) 2009 American Society for Mass Spectrometry

AB - Atmospheric pressure chemical ionization liquid chromatography-tandem mass spectrometry (APCI LC-MS/MS) of tetraether lipid cores of archaeal origin reveals distinct dissociation pathways for three classes of core lipid extracted from Methanobacter thermautotrophicus. Within these classes, two isobaric tetraether lipids, one a scarcely reported lipid constituent of M. thermautotrophicus and the other an artefact formed during extraction from cultured cells, were identified and distinguished via their MS2 spectra. APCI LC-MS/MS discriminates different tetraether core lipid types and isobaric species and reveals the mass of the constituent biphytanyl chains within the tetraether cores, albeit without full elucidation of their structures. Furthemore, the method allows direct estimation of the relative proportions of tetraether core lipids from chromatographic peak area measurement, allowing rapid profiling of these compounds in microbiological and environmental extracts. (J Am Soc Mass Spectrom 2009, 20, 51-59) (C) 2009 American Society for Mass Spectrometry

KW - METHANOBACTERIUM-THERMOAUTOTROPHICUM

KW - NAPHTHENATE DEPOSITION

KW - MEMBRANE-LIPIDS

KW - POLAR LIPIDS

KW - CRENARCHAEOL

KW - SEDIMENTS

KW - C-80

UR - http://www.scopus.com/inward/record.url?scp=57649210740&partnerID=8YFLogxK

U2 - 10.1016/j.jasms.2008.09.015

DO - 10.1016/j.jasms.2008.09.015

M3 - Article

SN - 1044-0305

VL - 20

SP - 51

EP - 59

JO - JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

JF - JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

IS - 1

ER -