Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

TY - JOUR

T1 - Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

AU - Flanagan, Lindsey A

AU - Wright, John J

AU - Roessler, Maxie M

AU - Moir, James W

AU - Parkin, Alison

N1 - © The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

PY - 2016/7/12

Y1 - 2016/7/12

N2 - Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

AB - Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

U2 - 10.1039/C6CC00515B

DO - 10.1039/C6CC00515B

M3 - Article

C2 - 27055899

SN - 1359-7345

VL - 52

SP - 9133

EP - 9136

JO - Chemical Communications

JF - Chemical Communications

IS - 58

ER -