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Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.
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Data in support of the paper: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance