Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

Lindsey A Flanagan, John J Wright, Maxie M Roessler, James W Moir, Alison Parkin

Research output: Contribution to journalArticlepeer-review


Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Original languageEnglish
Pages (from-to)9133-6
Number of pages4
JournalChemical Communications
Issue number58
Early online date31 Mar 2016
Publication statusPublished - 12 Jul 2016

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