@inproceedings{4bb33ae3e1574465921d26ac039bb631,
title = "Real-time, label-free mapping of cell signalling using photonic resonant imaging technology",
abstract = "Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro and real-time analysis of specific single-cell signalling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet de-sialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.",
keywords = "Biosensing, Cell secretion, Label-free, Photonics",
author = "Jos{\'e} Juan-Col{\'a}s and Hitchcock, {Ian S.} and Mark Coles and Steven Johnson and Krauss, {Thomas F.}",
year = "2018",
month = jan,
day = "1",
language = "English",
series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
publisher = "Chemical and Biological Microsystems Society",
pages = "1914--1916",
booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",
}