Real-time, label-free mapping of cell signalling using photonic resonant imaging technology

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Publication details

Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
DatePublished - 1 Jan 2018
Pages1914-1916
Number of pages3
PublisherChemical and Biological Microsystems Society
Original languageEnglish
ISBN (Electronic)9781510897571

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Abstract

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro and real-time analysis of specific single-cell signalling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet de-sialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

    Research areas

  • Biosensing, Cell secretion, Label-free, Photonics

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