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Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasite

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JournalMOLECULAR AND BIOCHEMICAL PARASITOLOGY
DateAccepted/In press - 15 Jun 2017
DateE-pub ahead of print - 16 Jun 2017
DatePublished (current) - Sep 2017
Volume216
Number of pages9
Pages (from-to)30-38
Early online date16/06/17
Original languageEnglish

Abstract

In this review we describe the expanding repertoire of molecular tools with which to study gene function in Leishmania. Specifically we review the tools available for studying functions of essential genes, such as plasmid shuffle and DiCre, as well as the rapidly expanding portfolio of available CRISPR/Cas9 approaches for large scale gene knockout and endogenous tagging. We include detail on approaches that allow the direct manipulation of RNA using RNAi and protein levels via Tet or DiCre induced overexpression and destabilization domain mediated degradation. The utilisation of current methods and the development of more advanced molecular tools will lead to greater understanding of the role of essential genes in the parasite and thereby more robust drug target validation, thereby paving the way for the development of novel therapeutics to treat this important disease.

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© 2017 The Authors

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  • Journal Article, Review

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