By the same authors

From the same journal

Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasite

Research output: Contribution to journalArticlepeer-review

Full text download(s)

Published copy (DOI)



Publication details

DateAccepted/In press - 15 Jun 2017
DateE-pub ahead of print - 16 Jun 2017
DatePublished (current) - Sep 2017
Number of pages9
Pages (from-to)30-38
Early online date16/06/17
Original languageEnglish


In this review we describe the expanding repertoire of molecular tools with which to study gene function in Leishmania. Specifically we review the tools available for studying functions of essential genes, such as plasmid shuffle and DiCre, as well as the rapidly expanding portfolio of available CRISPR/Cas9 approaches for large scale gene knockout and endogenous tagging. We include detail on approaches that allow the direct manipulation of RNA using RNAi and protein levels via Tet or DiCre induced overexpression and destabilization domain mediated degradation. The utilisation of current methods and the development of more advanced molecular tools will lead to greater understanding of the role of essential genes in the parasite and thereby more robust drug target validation, thereby paving the way for the development of novel therapeutics to treat this important disease.

Bibliographical note

© 2017 The Authors

    Research areas

  • Journal Article, Review

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations