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Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis

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Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis. / Lundqvist, A; Choudhury, A; Nagata, T; Andersson, T; Quinn, G; Fong, T; Maitland, N; Pettersson, S; Paulie, S; Pisa, P.

In: HUMAN GENE THERAPY, Vol. 13, No. 13, 09.2002, p. 1541-1549.

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Harvard

Lundqvist, A, Choudhury, A, Nagata, T, Andersson, T, Quinn, G, Fong, T, Maitland, N, Pettersson, S, Paulie, S & Pisa, P 2002, 'Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis', HUMAN GENE THERAPY, vol. 13, no. 13, pp. 1541-1549.

APA

Lundqvist, A., Choudhury, A., Nagata, T., Andersson, T., Quinn, G., Fong, T., ... Pisa, P. (2002). Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis. HUMAN GENE THERAPY, 13(13), 1541-1549.

Vancouver

Lundqvist A, Choudhury A, Nagata T, Andersson T, Quinn G, Fong T et al. Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis. HUMAN GENE THERAPY. 2002 Sep;13(13):1541-1549.

Author

Lundqvist, A ; Choudhury, A ; Nagata, T ; Andersson, T ; Quinn, G ; Fong, T ; Maitland, N ; Pettersson, S ; Paulie, S ; Pisa, P. / Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis. In: HUMAN GENE THERAPY. 2002 ; Vol. 13, No. 13. pp. 1541-1549.

Bibtex - Download

@article{f90368ec00a3499781c0663e6d266fa9,
title = "Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis",
abstract = "The aim of this study was to examine the effect of two of the most commonly used viral vectors, that is, retrovirus and adenovirus, on the antigen presentation of dendritic cells (DCs). DCs were generated from CD34(+) hematopoietic precursors and CD14(+) monocytes of the same prostate cancer patients. Adenoviral transduction of monocyte-derived DCs (MO-DCs) resulted in upregulation of CD80, CD86, and CD83 expression. Adenovirus-transduced MO-DCs were also more potent stimulators of allogeneic lymphocytes, produced increased amounts of the cytokines tumor necrosis factor alpha and interleukin 12 p70, and exhibited increased expression of NF-kappaB and antiapoptotic molecules Bcl-X-L and Bcl-2. Enhanced expression of the antiapoptotic molecules correlated with increased resistance of adenovirus-transduced MO-DCs to spontaneous as well as Fas-mediated cell death. In contrast to the adenoviral construct, no significant transduction of MO-DCs with the retrovirus could be obtained. Transduction of CD34(+) cell-derived DCs with the retrovirus or the adenovirus did not significantly alter expression of the costimulatory molecules or cytokines studied. At lower stimulation ratios, CD34(+) cell-derived DCs transduced with retrovirus were less potent in their ability to stimulate allogeneic lymphocytes in comparison with nontransduced DCs. Our results indicate that adenoviral vectors may be more suitable for gene delivery to DCs for immunotherapy.",
keywords = "GENE-TRANSFER, MEASLES-VIRUS, T-CELLS, MATURATION, INFECTION, DELIVERY, IMMUNOSUPPRESSION, INTERLEUKIN-12, EXPRESSION, INTEGRINS",
author = "A Lundqvist and A Choudhury and T Nagata and T Andersson and G Quinn and T Fong and N Maitland and S Pettersson and S Paulie and P Pisa",
year = "2002",
month = "9",
language = "English",
volume = "13",
pages = "1541--1549",
journal = "HUMAN GENE THERAPY",
issn = "1043-0342",
publisher = "Mary Ann Liebert Inc.",
number = "13",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Recombinant adenovirus vector activates and protects human monocyte-derived dendritic cells from apoptosis

AU - Lundqvist, A

AU - Choudhury, A

AU - Nagata, T

AU - Andersson, T

AU - Quinn, G

AU - Fong, T

AU - Maitland, N

AU - Pettersson, S

AU - Paulie, S

AU - Pisa, P

PY - 2002/9

Y1 - 2002/9

N2 - The aim of this study was to examine the effect of two of the most commonly used viral vectors, that is, retrovirus and adenovirus, on the antigen presentation of dendritic cells (DCs). DCs were generated from CD34(+) hematopoietic precursors and CD14(+) monocytes of the same prostate cancer patients. Adenoviral transduction of monocyte-derived DCs (MO-DCs) resulted in upregulation of CD80, CD86, and CD83 expression. Adenovirus-transduced MO-DCs were also more potent stimulators of allogeneic lymphocytes, produced increased amounts of the cytokines tumor necrosis factor alpha and interleukin 12 p70, and exhibited increased expression of NF-kappaB and antiapoptotic molecules Bcl-X-L and Bcl-2. Enhanced expression of the antiapoptotic molecules correlated with increased resistance of adenovirus-transduced MO-DCs to spontaneous as well as Fas-mediated cell death. In contrast to the adenoviral construct, no significant transduction of MO-DCs with the retrovirus could be obtained. Transduction of CD34(+) cell-derived DCs with the retrovirus or the adenovirus did not significantly alter expression of the costimulatory molecules or cytokines studied. At lower stimulation ratios, CD34(+) cell-derived DCs transduced with retrovirus were less potent in their ability to stimulate allogeneic lymphocytes in comparison with nontransduced DCs. Our results indicate that adenoviral vectors may be more suitable for gene delivery to DCs for immunotherapy.

AB - The aim of this study was to examine the effect of two of the most commonly used viral vectors, that is, retrovirus and adenovirus, on the antigen presentation of dendritic cells (DCs). DCs were generated from CD34(+) hematopoietic precursors and CD14(+) monocytes of the same prostate cancer patients. Adenoviral transduction of monocyte-derived DCs (MO-DCs) resulted in upregulation of CD80, CD86, and CD83 expression. Adenovirus-transduced MO-DCs were also more potent stimulators of allogeneic lymphocytes, produced increased amounts of the cytokines tumor necrosis factor alpha and interleukin 12 p70, and exhibited increased expression of NF-kappaB and antiapoptotic molecules Bcl-X-L and Bcl-2. Enhanced expression of the antiapoptotic molecules correlated with increased resistance of adenovirus-transduced MO-DCs to spontaneous as well as Fas-mediated cell death. In contrast to the adenoviral construct, no significant transduction of MO-DCs with the retrovirus could be obtained. Transduction of CD34(+) cell-derived DCs with the retrovirus or the adenovirus did not significantly alter expression of the costimulatory molecules or cytokines studied. At lower stimulation ratios, CD34(+) cell-derived DCs transduced with retrovirus were less potent in their ability to stimulate allogeneic lymphocytes in comparison with nontransduced DCs. Our results indicate that adenoviral vectors may be more suitable for gene delivery to DCs for immunotherapy.

KW - GENE-TRANSFER

KW - MEASLES-VIRUS

KW - T-CELLS

KW - MATURATION

KW - INFECTION

KW - DELIVERY

KW - IMMUNOSUPPRESSION

KW - INTERLEUKIN-12

KW - EXPRESSION

KW - INTEGRINS

M3 - Article

VL - 13

SP - 1541

EP - 1549

JO - HUMAN GENE THERAPY

T2 - HUMAN GENE THERAPY

JF - HUMAN GENE THERAPY

SN - 1043-0342

IS - 13

ER -