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Recombinant portal protein from Staphylococcus epidermidis bacteriophage CNPH82 is a 13-subunit oligomer

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JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
DatePublished - 1 Oct 2012
Issue numberPt 10
Volume68
Number of pages4
Pages (from-to)1267-1270
Original languageEnglish

Abstract

The portal protein cn3 of bacteriophage CNPH82 is predicted to serve as a gateway for translocation of viral genome into preformed pro-capsid, like portal proteins from other double-stranded DNA tailed bacteriophages. The host of bacteriophage CNPH82 is the opportunistic human pathogenic bacterium Staphylococcus epidermidis, a major cause of nosocomial infections. The portal protein of this phage has been cloned, overexpressed and purified. Size-exclusion chromatography-multi-angle laser light scattering analysis has indicated that the portal protein contains ∼13 subunits. Crystals of the portal protein, diffracting to 4.2 Å, have been obtained. These crystals belong to the space group C222(1) with the unit-cell parameters of a = 252.4, b = 367.0, c = 175.5 Å. The self-rotation function revealed the presence of a single 13-subunit oligomer in the asymmetric unit.

    Research areas

  • Protein Subunits, Recombinant Proteins, Staphylococcus Phages, Staphylococcus epidermidis, Viral Proteins

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