Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

By the same authors

Conserved Histidine Adjacent to the Proximal Cluster Tunes the Anaerobic Reductive Activation of Escherichia coli Membrane-Bound [NiFe] Hydrogenase-1
Flanagan, L. A., Chidwick, H., Walton, J., Moir, J. & Parkin, A., 16 Feb 2018
Article in ChemElectroChem
Using Purely Sinusoidal Voltammetry for Rapid Inference of Surface-Confined Electrochemical Reaction Parameters
Lloyd-Laney, H. O., Yates, N. D. J., Robinson, M. J., Hewson, A. R., Firth, J. D., Elton, D. M., Zhang, J., Bond, A. M., Parkin, A. & Gavaghan, D. J., 8 Jan 2021
Article in Analytical Chemistry
Photocatalytic Deoxygenation of Sulfoxides Using Visible Light: Mechanistic Investigations and Synthetic Applications
Clarke, A. K., Parkin, A., Taylor, R. J. K., Unsworth, W. P. & Rossi-Ashton, J. A., 15 May 2020
Article in ACS Catalysis
The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus
Coombes, D., Davies, J. S., Newton-Vesty, M. C., Horne, C. R., Setty, T. G., Subramanian, R., Moir, J. W. B., Friemann, R., Panjikar, S., Griffin, M. D. W., North, R. A. & Dobson, R. C. J., 6 Mar 2020
Article in The Journal of biological chemistry
Genomic basis for pesticide degradation revealed by selection, isolation and characterisation of a library of metaldehyde-degrading strains from soil
Castro Gutiérrez, V., Fuller, E., Thomas, J. C., Sinclair, C. J., Johnson, S. D., Helgason, T. & Moir, J., 1 Mar 2020
Article in Soil Biology and Biochemistry

From the same journal

The Impact of the Counterion in the Performance of Ionic Hydrotropes
Abranches, D. O., Benfica, J., Soares, B., Ana M, F., Sintra, T. E., Shimizu, S. & Coutinho, J. A. P., 17 Feb 2021, (Accepted/In press)
Article in Chemical Communications
Structural insights into heparanase activity using a fluorogenic heparan sulfate disaccharide
Wu, L., Wimmer, N., Davies, G. J. & Ferro, V., 18 Nov 2020
Article in Chemical Communications
Two-component supramolecular hydrogel for controlled drug release
Patterson, A. & Smith, D. K., 18 Aug 2020
Article in Chemical communications
Photo-patterned multi-domain multi-component hybrid hydrogels
Cornwell, D. & Smith, D. K., 26 May 2020
Article in Chemical communications
Unveiling the mechanism of hydrotropy: evidence for water-mediated aggregations of hydrotrope around the solute
Abranches, D. O., Benfica, J., Soares, B. P., Leal-Duaso, A., Sintra, T. E., Pires, E., Pinho, S. P., Shimizu, S. & Coutinho, J. A. P., 21 May 2020
Article in Chemical Communications

From the same journal

Chemical communications (Journal)
Lianne Irene Willems (Reviewer)
29 Jun 2019 → 11 Oct 2019
Activity: Publication peer-review and editorial work › Journal peer review

Research output: Contribution to journal › Article › peer-review

Full text download(s)

ChemComm_Parkin_Submission_correction_FINALVERSION_black
629 KB, PDF document

Published copy (DOI)

10.1039/C6CC00515B

Author(s)

Lindsey A Flanagan
John J Wright
Maxie M Roessler
James W Moir
Alison Parkin

Department/unit(s)

Publication details

Journal	Chemical Communications
Date	Accepted/In press - 31 Mar 2016
Date	E-pub ahead of print - 31 Mar 2016
Date	Published (current) - 12 Jul 2016
Issue number	58
Volume	52
Number of pages	4
Pages (from-to)	9133-6
Early online date	31/03/16
Original language	English

Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Bibliographical note

© The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations

York staff: edit these data

The York Research Database

By the same authors

Conserved Histidine Adjacent to the Proximal Cluster Tunes the Anaerobic Reductive Activation of Escherichia coli Membrane-Bound [NiFe] Hydrogenase-1

Using Purely Sinusoidal Voltammetry for Rapid Inference of Surface-Confined Electrochemical Reaction Parameters

Photocatalytic Deoxygenation of Sulfoxides Using Visible Light: Mechanistic Investigations and Synthetic Applications

The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus

Genomic basis for pesticide degradation revealed by selection, isolation and characterisation of a library of metaldehyde-degrading strains from soil

From the same journal

The Impact of the Counterion in the Performance of Ionic Hydrotropes

Structural insights into heparanase activity using a fluorogenic heparan sulfate disaccharide

Two-component supramolecular hydrogel for controlled drug release

Photo-patterned multi-domain multi-component hybrid hydrogels

Unveiling the mechanism of hydrotropy: evidence for water-mediated aggregations of hydrotrope around the solute

From the same journal

Chemical communications (Journal)