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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

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JournalChemical Communications
DateAccepted/In press - 31 Mar 2016
DateE-pub ahead of print - 31 Mar 2016
DatePublished (current) - 12 Jul 2016
Issue number58
Volume52
Number of pages4
Pages (from-to)9133-6
Early online date31/03/16
Original languageEnglish

Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

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© The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

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