Regioselective glucosylation of aromatic compounds: screening of a recombinant Glycosyltransferase library to identify new biocatalysts

M Weis; E K Lim; N Bruce; D Bowles

doi:10.1002/anie.200504505

Regioselective glucosylation of aromatic compounds: screening of a recombinant Glycosyltransferase library to identify new biocatalysts

M Weis, E K Lim, N Bruce, D Bowles

CNAP

Research output: Contribution to journal › Article › peer-review

Abstract

A novel whole-cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D-glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D-glucose to trans-resveratrol were identified that have the regioselectivity shown in the picture.

Original language	English
Pages (from-to)	3534-3538
Number of pages	4
Journal	Angewandte Chemie International Edition
Volume	45
Issue number	21
DOIs	https://doi.org/10.1002/anie.200504505
Publication status	Published - 24 Apr 2006

Keywords

enzymes
glycosides
glycosylation
regioselectivity
screening
LARGE-SCALE PRODUCTION
ARABIDOPSIS-THALIANA
PLANT GLYCOSYLTRANSFERASES
ENGINEERED BACTERIA
ESTROGEN-RECEPTOR
MULTIGENE FAMILY
ASSAY
RESVERATROL
ENZYMES
ISOFLAVONES

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10.1002/anie.200504505

http://dx.doi.org/10.1002/anie.200504505

Exploiting genomics to make glycosidic bonds in vitro and metabolic engineering in vivo
Bowles, D. J. (Principal investigator)
BBSRC
2/09/02 → 30/11/07
Project: Research project (funded) › Research

Cite this

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title = "Regioselective glucosylation of aromatic compounds: screening of a recombinant Glycosyltransferase library to identify new biocatalysts",

abstract = "A novel whole-cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D-glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D-glucose to trans-resveratrol were identified that have the regioselectivity shown in the picture.",

keywords = "enzymes, glycosides, glycosylation, regioselectivity, screening, LARGE-SCALE PRODUCTION, ARABIDOPSIS-THALIANA, PLANT GLYCOSYLTRANSFERASES, ENGINEERED BACTERIA, ESTROGEN-RECEPTOR, MULTIGENE FAMILY, ASSAY, RESVERATROL, ENZYMES, ISOFLAVONES",

author = "M Weis and Lim, {E K} and N Bruce and D Bowles",

year = "2006",

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day = "24",

doi = "10.1002/anie.200504505",

language = "English",

volume = "45",

pages = "3534--3538",

journal = "Angewandte Chemie International Edition",

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T1 - Regioselective glucosylation of aromatic compounds: screening of a recombinant Glycosyltransferase library to identify new biocatalysts

AU - Weis, M

AU - Lim, E K

AU - Bruce, N

AU - Bowles, D

PY - 2006/4/24

Y1 - 2006/4/24

N2 - A novel whole-cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D-glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D-glucose to trans-resveratrol were identified that have the regioselectivity shown in the picture.

AB - A novel whole-cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D-glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D-glucose to trans-resveratrol were identified that have the regioselectivity shown in the picture.

KW - enzymes

KW - glycosides

KW - glycosylation

KW - regioselectivity

KW - screening

KW - LARGE-SCALE PRODUCTION

KW - ARABIDOPSIS-THALIANA

KW - PLANT GLYCOSYLTRANSFERASES

KW - ENGINEERED BACTERIA

KW - ESTROGEN-RECEPTOR

KW - MULTIGENE FAMILY

KW - ASSAY

KW - RESVERATROL

KW - ENZYMES

KW - ISOFLAVONES

U2 - 10.1002/anie.200504505

DO - 10.1002/anie.200504505

M3 - Article

SN - 1433-7851

VL - 45

SP - 3534

EP - 3538

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 21

ER -

Regioselective glucosylation of aromatic compounds: screening of a recombinant Glycosyltransferase library to identify new biocatalysts

Abstract

Keywords

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Projects

Exploiting genomics to make glycosidic bonds in vitro and metabolic engineering in vivo

Cite this