Removal of t(1) noise from metabolomic 2D (1)H-(13)C HSQC NMR spectra by Correlated Trace Denoising

Simon Poulding; Adrian J. Charlton; James Donarski; Julie C. Wilson

doi:DOI: 10.1016/j.jmr.2007.09.004

Removal of t(1) noise from metabolomic 2D (1)H-(13)C HSQC NMR spectra by Correlated Trace Denoising

Simon Poulding, Adrian J. Charlton, James Donarski, Julie C. Wilson

Research output: Contribution to journal › Article › peer-review

Abstract

The presence of t1 noise artefacts in 2D phase-cycled Heteronuclear Single Quantum Coherence (HSQC) spectra constrains the use of this experiment despite its superior sensitivity. This paper proposes a new processing algorithm, working in the frequency-domain, for reducing t1 noise. The algorithm has been developed for use in contexts, such as metabolomic studies, where existing denoising techniques cannot always be applied. Two test cases are presented that show the algorithm to be effective in improving the SNR of peaks embedded within t1 noise by a factor of more than 2, while retaining the intensity and shape of genuine peaks.

Keywords: 2D NMR Spectroscopy; HSQC; t1 noise; Trace correlation; Metabolomics

Original language	Undefined/Unknown
Pages (from-to)	190-199
Number of pages	99
Journal	J. Magn. Reson.
Volume	189
Issue number	2
DOIs	https://doi.org/DOI: 10.1016/j.jmr.2007.09.004
Publication status	Published - 2007

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DOI: 10.1016/j.jmr.2007.09.004

http://www.sciencedirect.com/science/article/pii/S1090780707002704

Cite this

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title = "Removal of t(1) noise from metabolomic 2D (1)H-(13)C HSQC NMR spectra by Correlated Trace Denoising",

abstract = "The presence of t1 noise artefacts in 2D phase-cycled Heteronuclear Single Quantum Coherence (HSQC) spectra constrains the use of this experiment despite its superior sensitivity. This paper proposes a new processing algorithm, working in the frequency-domain, for reducing t1 noise. The algorithm has been developed for use in contexts, such as metabolomic studies, where existing denoising techniques cannot always be applied. Two test cases are presented that show the algorithm to be effective in improving the SNR of peaks embedded within t1 noise by a factor of more than 2, while retaining the intensity and shape of genuine peaks.Keywords: 2D NMR Spectroscopy; HSQC; t1 noise; Trace correlation; Metabolomics",

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T1 - Removal of t(1) noise from metabolomic 2D (1)H-(13)C HSQC NMR spectra by Correlated Trace Denoising

AU - Poulding, Simon

AU - Charlton, Adrian J.

AU - Donarski, James

AU - Wilson, Julie C.

PY - 2007

Y1 - 2007

N2 - The presence of t1 noise artefacts in 2D phase-cycled Heteronuclear Single Quantum Coherence (HSQC) spectra constrains the use of this experiment despite its superior sensitivity. This paper proposes a new processing algorithm, working in the frequency-domain, for reducing t1 noise. The algorithm has been developed for use in contexts, such as metabolomic studies, where existing denoising techniques cannot always be applied. Two test cases are presented that show the algorithm to be effective in improving the SNR of peaks embedded within t1 noise by a factor of more than 2, while retaining the intensity and shape of genuine peaks.Keywords: 2D NMR Spectroscopy; HSQC; t1 noise; Trace correlation; Metabolomics

AB - The presence of t1 noise artefacts in 2D phase-cycled Heteronuclear Single Quantum Coherence (HSQC) spectra constrains the use of this experiment despite its superior sensitivity. This paper proposes a new processing algorithm, working in the frequency-domain, for reducing t1 noise. The algorithm has been developed for use in contexts, such as metabolomic studies, where existing denoising techniques cannot always be applied. Two test cases are presented that show the algorithm to be effective in improving the SNR of peaks embedded within t1 noise by a factor of more than 2, while retaining the intensity and shape of genuine peaks.Keywords: 2D NMR Spectroscopy; HSQC; t1 noise; Trace correlation; Metabolomics

U2 - DOI: 10.1016/j.jmr.2007.09.004

DO - DOI: 10.1016/j.jmr.2007.09.004

M3 - Article

VL - 189

SP - 190

EP - 199

JO - J. Magn. Reson.

JF - J. Magn. Reson.

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