Respiration of the external mycelium in the arbuscular mycorrhizal symbiosis shows strong dependence on recent photosynthates and acclimation to temperature

A Heinemeyer; P Ineson; N Ostle; A H Fitter

doi:10.1111/j.1469-8137.2006.01730.x

Respiration of the external mycelium in the arbuscular mycorrhizal symbiosis shows strong dependence on recent photosynthates and acclimation to temperature

A Heinemeyer, P Ineson, N Ostle, A H Fitter

Research output: Contribution to journal › Article › peer-review

Abstract

Although arbuscular mycorrhizal (AM) fungi are a major pathway in the global carbon cycle, their basic biology and, in particular, their respiratory response to temperature remain obscure.

A pulse label of the stable isotope C-13 was applied to Plantago lanceolata, either uninoculated or inoculated with the AM fungus Glomus mosseae. The extra-radical mycelium (ERM) of the fungus was allowed to grow into a separate hyphal compartment excluding roots. We determined the carbon costs of the ERM and tested for a direct temperature effect on its respiration by measuring total carbon and the C-13:C-12 ratio of respired CO2. With a second pulse we tested for acclimation of ERM respiration after 2 wk of soil warming.

Root colonization remained unchanged between the two pulses but warming the hyphal compartment increased ERM length. delta C-13 signals peaked within the first 10 h and were higher in mycorrhizal treatments. The concentration of CO2 in the gas samples fluctuated diurnally and was highest in the mycorrhizal treatments but was unaffected by temperature. Heating increased ERM respiration only after the first pulse and reduced specific ERM respiration rates after the second pulse; however, both pulses strongly depended on radiation flux.

The results indicate a fast ERM acclimation to temperature, and that light is the key factor controlling carbon allocation to the fungus.

Original language	English
Pages (from-to)	159-170
Number of pages	12
Journal	New Phytologist
Volume	171
Issue number	1
DOIs	https://doi.org/10.1111/j.1469-8137.2006.01730.x
Publication status	Published - 2006

Bibliographical note

Open access copy available from the journal web site.

Keywords

arbuscular mycorrhiza (AM)
below-ground carbon allocation
C-13 label
compartment experiment
mycelial growth
mycelial respiration
Plantago lanceolata
temperature acclimation of respiration
ATMOSPHERIC CARBON-DIOXIDE
TRIFOLIUM-SUBTERRANEUM L
PLANTAGO-LANCEOLATA
FUNCTIONAL DIVERSITY
ECOSYSTEM RESPONSES
PHOSPHORUS INFLOW
CO2 CONCENTRATION
GLOMUS-MOSSEAE
CLIMATE-CHANGE
ELEVATED CO2

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10.1111/j.1469-8137.2006.01730.x

http://dx.doi.org/10.1111/j.1469-8137.2006.01730.x

Cite this

@article{a94d111786404a098197bd0f3b9fa62f,

title = "Respiration of the external mycelium in the arbuscular mycorrhizal symbiosis shows strong dependence on recent photosynthates and acclimation to temperature",

abstract = "Although arbuscular mycorrhizal (AM) fungi are a major pathway in the global carbon cycle, their basic biology and, in particular, their respiratory response to temperature remain obscure.A pulse label of the stable isotope C-13 was applied to Plantago lanceolata, either uninoculated or inoculated with the AM fungus Glomus mosseae. The extra-radical mycelium (ERM) of the fungus was allowed to grow into a separate hyphal compartment excluding roots. We determined the carbon costs of the ERM and tested for a direct temperature effect on its respiration by measuring total carbon and the C-13:C-12 ratio of respired CO2. With a second pulse we tested for acclimation of ERM respiration after 2 wk of soil warming.Root colonization remained unchanged between the two pulses but warming the hyphal compartment increased ERM length. delta C-13 signals peaked within the first 10 h and were higher in mycorrhizal treatments. The concentration of CO2 in the gas samples fluctuated diurnally and was highest in the mycorrhizal treatments but was unaffected by temperature. Heating increased ERM respiration only after the first pulse and reduced specific ERM respiration rates after the second pulse; however, both pulses strongly depended on radiation flux.The results indicate a fast ERM acclimation to temperature, and that light is the key factor controlling carbon allocation to the fungus.",

keywords = "arbuscular mycorrhiza (AM), below-ground carbon allocation, C-13 label, compartment experiment, mycelial growth, mycelial respiration, Plantago lanceolata, temperature acclimation of respiration, ATMOSPHERIC CARBON-DIOXIDE, TRIFOLIUM-SUBTERRANEUM L, PLANTAGO-LANCEOLATA, FUNCTIONAL DIVERSITY, ECOSYSTEM RESPONSES, PHOSPHORUS INFLOW, CO2 CONCENTRATION, GLOMUS-MOSSEAE, CLIMATE-CHANGE, ELEVATED CO2",

author = "A Heinemeyer and P Ineson and N Ostle and Fitter, {A H}",

note = "Open access copy available from the journal web site.",

year = "2006",

doi = "10.1111/j.1469-8137.2006.01730.x",

language = "English",

volume = "171",

pages = "159--170",

journal = "New Phytologist",

issn = "0028-646X",

publisher = "Wiley-Blackwell",

number = "1",

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TY - JOUR

T1 - Respiration of the external mycelium in the arbuscular mycorrhizal symbiosis shows strong dependence on recent photosynthates and acclimation to temperature

AU - Heinemeyer, A

AU - Ineson, P

AU - Ostle, N

AU - Fitter, A H

N1 - Open access copy available from the journal web site.

PY - 2006

Y1 - 2006

N2 - Although arbuscular mycorrhizal (AM) fungi are a major pathway in the global carbon cycle, their basic biology and, in particular, their respiratory response to temperature remain obscure.A pulse label of the stable isotope C-13 was applied to Plantago lanceolata, either uninoculated or inoculated with the AM fungus Glomus mosseae. The extra-radical mycelium (ERM) of the fungus was allowed to grow into a separate hyphal compartment excluding roots. We determined the carbon costs of the ERM and tested for a direct temperature effect on its respiration by measuring total carbon and the C-13:C-12 ratio of respired CO2. With a second pulse we tested for acclimation of ERM respiration after 2 wk of soil warming.Root colonization remained unchanged between the two pulses but warming the hyphal compartment increased ERM length. delta C-13 signals peaked within the first 10 h and were higher in mycorrhizal treatments. The concentration of CO2 in the gas samples fluctuated diurnally and was highest in the mycorrhizal treatments but was unaffected by temperature. Heating increased ERM respiration only after the first pulse and reduced specific ERM respiration rates after the second pulse; however, both pulses strongly depended on radiation flux.The results indicate a fast ERM acclimation to temperature, and that light is the key factor controlling carbon allocation to the fungus.

AB - Although arbuscular mycorrhizal (AM) fungi are a major pathway in the global carbon cycle, their basic biology and, in particular, their respiratory response to temperature remain obscure.A pulse label of the stable isotope C-13 was applied to Plantago lanceolata, either uninoculated or inoculated with the AM fungus Glomus mosseae. The extra-radical mycelium (ERM) of the fungus was allowed to grow into a separate hyphal compartment excluding roots. We determined the carbon costs of the ERM and tested for a direct temperature effect on its respiration by measuring total carbon and the C-13:C-12 ratio of respired CO2. With a second pulse we tested for acclimation of ERM respiration after 2 wk of soil warming.Root colonization remained unchanged between the two pulses but warming the hyphal compartment increased ERM length. delta C-13 signals peaked within the first 10 h and were higher in mycorrhizal treatments. The concentration of CO2 in the gas samples fluctuated diurnally and was highest in the mycorrhizal treatments but was unaffected by temperature. Heating increased ERM respiration only after the first pulse and reduced specific ERM respiration rates after the second pulse; however, both pulses strongly depended on radiation flux.The results indicate a fast ERM acclimation to temperature, and that light is the key factor controlling carbon allocation to the fungus.

KW - arbuscular mycorrhiza (AM)

KW - below-ground carbon allocation

KW - C-13 label

KW - compartment experiment

KW - mycelial growth

KW - mycelial respiration

KW - Plantago lanceolata

KW - temperature acclimation of respiration

KW - ATMOSPHERIC CARBON-DIOXIDE

KW - TRIFOLIUM-SUBTERRANEUM L

KW - PLANTAGO-LANCEOLATA

KW - FUNCTIONAL DIVERSITY

KW - ECOSYSTEM RESPONSES

KW - PHOSPHORUS INFLOW

KW - CO2 CONCENTRATION

KW - GLOMUS-MOSSEAE

KW - CLIMATE-CHANGE

KW - ELEVATED CO2

U2 - 10.1111/j.1469-8137.2006.01730.x

DO - 10.1111/j.1469-8137.2006.01730.x

M3 - Article

SN - 0028-646X

VL - 171

SP - 159

EP - 170

JO - New Phytologist

JF - New Phytologist

IS - 1

ER -