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Response of cell wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus

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Author(s)

  • Lucy Sarah Hyde
  • Till K. Pellny
  • Jackie Freeman
  • Louise V. Michaelson
  • Rachael Simister
  • Simon John McQueen Mason
  • Rowan A.C. Mitchell

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Publication details

JournalPlanta
DateAccepted/In press - 26 Jul 2018
DateE-pub ahead of print - 9 Aug 2018
DatePublished (current) - 1 Nov 2018
Issue number5
Volume248
Number of pages17
Pages (from-to)1213-1229
Early online date9/08/18
Original languageEnglish

Abstract

Main Conclusion: Methyl-jasmonate induces large increases in p-coumarate linked to arabinoxylan in Brachypodium and in abundance of GT61 and BAHD family transcripts consistent with a role in synthesis of this linkage. Jasmonic acid (JA) signalling is required for many stress responses in plants, inducing large changes in the transcriptome, including up-regulation of transcripts associated with lignifcation. However, less is known about the response to JA of grass cell walls and the monocot-specifc features of arabinoxylan (AX) synthesis and acylation by ferulic acid (FA) and paracoumaric acid (pCA). Here, we show that methyl-jasmonate (MeJA) induces moderate increases in FA monomer,>50% increases in FA dimers, and fve–sixfold increases in pCA ester-linked to cell walls in Brachypodium callus. Direct measurement of arabinose acylated by pCA (Araf-pCA) indicated that most or all the increase in cell-wall pCA was due to pCA ester-linked to AX. Analysis of the RNA-seq transcriptome of the callus response showed that these cell-wall changes were accompanied by up-regulation of members of the GT61 and BAHD gene families implicated in AX decoration and acylation; two BAHD paralogues were among the most up-regulated cell-wall genes (seven and fvefold) after 24 h exposure to MeJA. Similar responses to JA of orthologous BAHD and GT61 transcripts are present in the RiceXPro public expression data set for rice seedlings, showing that they are not specifc to Brachypodium or to callus. The large response of AX-pCA to MeJA may, therefore, indicate an important role for this linkage in response of primary cell walls of grasses to JA signalling

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© The Author(s) 2018

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