Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source

Ignacio Moya Ramírez; Konstantina Tsaousi; Michelle Rudden; Roger Marchant; Encarnación Jurado Alameda; Miguel García Román; Ibrahim M Banat

doi:10.1016/j.biortech.2015.09.012

Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source

Ignacio Moya Ramírez, Konstantina Tsaousi, Michelle Rudden, Roger Marchant, Encarnación Jurado Alameda, Miguel García Román, Ibrahim M Banat

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Olive mill waste (OMW) creates a major environmental problem due to the difficulty of further waste processing. In this work we present an approach to give OMW added value by using it for the production of biosurfactants. Two bacterial species, Pseudomonas aeruginosa and Bacillus subtilis, were grown with OMW as the sole carbon source. Glycerol and waste frying oil were used as comparative carbon sources. B. subtilis produced surfactin (a lipopeptide) at a maximum concentration of 3.12 mg/L with 2% w/v of OMW in the medium, dropping to 0.57 mg/L with 10% w/v of OMW. In contrast, P. aeruginosa produced 8.78 mg/L of rhamnolipid with 2% w/v OMW increasing to 191.46 mg/L with 10% w/v OMW. The use of solvent-extracted OMW reduced the biosurfactant production by 70.8% and 88.3% for B. subtilis and P. aeruginosa respectively. These results confirm that OMW is a potential substrate for biosurfactant production.

Original language	English
Pages (from-to)	231-236
Journal	BIORESOURCE TECHNOLOGY
Volume	198
DOIs	https://doi.org/10.1016/j.biortech.2015.09.012
Publication status	Published - 1 Dec 2015

Bibliographical note

Keywords

Bacillus subtilis
Carbon/metabolism
Glycolipids/biosynthesis
Lipopeptides/biosynthesis
Olive Oil/metabolism
Peptides, Cyclic/biosynthesis
Plant Oils
Pseudomonas aeruginosa

Access to Document

10.1016/j.biortech.2015.09.012

Cite this

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title = "Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source",

abstract = "Olive mill waste (OMW) creates a major environmental problem due to the difficulty of further waste processing. In this work we present an approach to give OMW added value by using it for the production of biosurfactants. Two bacterial species, Pseudomonas aeruginosa and Bacillus subtilis, were grown with OMW as the sole carbon source. Glycerol and waste frying oil were used as comparative carbon sources. B. subtilis produced surfactin (a lipopeptide) at a maximum concentration of 3.12 mg/L with 2% w/v of OMW in the medium, dropping to 0.57 mg/L with 10% w/v of OMW. In contrast, P. aeruginosa produced 8.78 mg/L of rhamnolipid with 2% w/v OMW increasing to 191.46 mg/L with 10% w/v OMW. The use of solvent-extracted OMW reduced the biosurfactant production by 70.8% and 88.3% for B. subtilis and P. aeruginosa respectively. These results confirm that OMW is a potential substrate for biosurfactant production.",

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author = "{Moya Ram{\'i}rez}, Ignacio and Konstantina Tsaousi and Michelle Rudden and Roger Marchant and {Jurado Alameda}, Encarnaci{\'o}n and {Garc{\'i}a Rom{\'a}n}, Miguel and Banat, {Ibrahim M}",

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AU - Moya Ramírez, Ignacio

AU - Tsaousi, Konstantina

AU - Rudden, Michelle

AU - Marchant, Roger

AU - Jurado Alameda, Encarnación

AU - García Román, Miguel

AU - Banat, Ibrahim M

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Olive mill waste (OMW) creates a major environmental problem due to the difficulty of further waste processing. In this work we present an approach to give OMW added value by using it for the production of biosurfactants. Two bacterial species, Pseudomonas aeruginosa and Bacillus subtilis, were grown with OMW as the sole carbon source. Glycerol and waste frying oil were used as comparative carbon sources. B. subtilis produced surfactin (a lipopeptide) at a maximum concentration of 3.12 mg/L with 2% w/v of OMW in the medium, dropping to 0.57 mg/L with 10% w/v of OMW. In contrast, P. aeruginosa produced 8.78 mg/L of rhamnolipid with 2% w/v OMW increasing to 191.46 mg/L with 10% w/v OMW. The use of solvent-extracted OMW reduced the biosurfactant production by 70.8% and 88.3% for B. subtilis and P. aeruginosa respectively. These results confirm that OMW is a potential substrate for biosurfactant production.

AB - Olive mill waste (OMW) creates a major environmental problem due to the difficulty of further waste processing. In this work we present an approach to give OMW added value by using it for the production of biosurfactants. Two bacterial species, Pseudomonas aeruginosa and Bacillus subtilis, were grown with OMW as the sole carbon source. Glycerol and waste frying oil were used as comparative carbon sources. B. subtilis produced surfactin (a lipopeptide) at a maximum concentration of 3.12 mg/L with 2% w/v of OMW in the medium, dropping to 0.57 mg/L with 10% w/v of OMW. In contrast, P. aeruginosa produced 8.78 mg/L of rhamnolipid with 2% w/v OMW increasing to 191.46 mg/L with 10% w/v OMW. The use of solvent-extracted OMW reduced the biosurfactant production by 70.8% and 88.3% for B. subtilis and P. aeruginosa respectively. These results confirm that OMW is a potential substrate for biosurfactant production.

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