Rhizobium etli is the dominant common bean nodulating rhizobia in cultivated soils from different locations in Jordan

S A Tamimi; J P W Young

doi:10.1016/j.apsoil.2004.01.003

Rhizobium etli is the dominant common bean nodulating rhizobia in cultivated soils from different locations in Jordan

S A Tamimi, J P W Young

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Thirty rhizobial isolates that nodulate common bean (Phaseolus vulgaris L.) plants were obtained from a range of cultivated soils covering 16 geographical sites in Jordan. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for nodA, nifH and g7nII genes and sequencing methods showed that the rhizobial isolates formed two main groups. The first, which makes up 80% of isolates, were identified as Rhizobium etli while the second group comprising the remaining 20% were related to Rhizobium tropici. These findings indicate that R. etli is the predominant rhizobium that nodulates common bean in Jordan. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	193-200
Number of pages	8
Journal	Applied Soil Ecology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1016/j.apsoil.2004.01.003
Publication status	Published - Jul 2004

Keywords

N-2 fixation
Phaseolus valgaris
R. etli
R. tropici
PCR-RFLP
genotyping
PHASEOLUS-VULGARIS L
GENETIC DIVERSITY
NIFH GENES
NODULES
STRAINS
TROPICI
AFRICA
TREES
FIELD

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10.1016/j.apsoil.2004.01.003

Cite this

@article{f886c32818f64c0f97ad5cc57771d775,

title = "Rhizobium etli is the dominant common bean nodulating rhizobia in cultivated soils from different locations in Jordan",

abstract = "Thirty rhizobial isolates that nodulate common bean (Phaseolus vulgaris L.) plants were obtained from a range of cultivated soils covering 16 geographical sites in Jordan. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for nodA, nifH and g7nII genes and sequencing methods showed that the rhizobial isolates formed two main groups. The first, which makes up 80% of isolates, were identified as Rhizobium etli while the second group comprising the remaining 20% were related to Rhizobium tropici. These findings indicate that R. etli is the predominant rhizobium that nodulates common bean in Jordan. (C) 2004 Elsevier B.V. All rights reserved.",

keywords = "N-2 fixation, Phaseolus valgaris, R. etli, R. tropici, PCR-RFLP, genotyping, PHASEOLUS-VULGARIS L, GENETIC DIVERSITY, NIFH GENES, NODULES, STRAINS, TROPICI, AFRICA, TREES, FIELD",

author = "Tamimi, {S A} and Young, {J P W}",

year = "2004",

month = jul,

doi = "10.1016/j.apsoil.2004.01.003",

language = "English",

volume = "26",

pages = "193--200",

journal = "Applied Soil Ecology",

issn = "0929-1393",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Rhizobium etli is the dominant common bean nodulating rhizobia in cultivated soils from different locations in Jordan

AU - Tamimi, S A

AU - Young, J P W

PY - 2004/7

Y1 - 2004/7

N2 - Thirty rhizobial isolates that nodulate common bean (Phaseolus vulgaris L.) plants were obtained from a range of cultivated soils covering 16 geographical sites in Jordan. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for nodA, nifH and g7nII genes and sequencing methods showed that the rhizobial isolates formed two main groups. The first, which makes up 80% of isolates, were identified as Rhizobium etli while the second group comprising the remaining 20% were related to Rhizobium tropici. These findings indicate that R. etli is the predominant rhizobium that nodulates common bean in Jordan. (C) 2004 Elsevier B.V. All rights reserved.

AB - Thirty rhizobial isolates that nodulate common bean (Phaseolus vulgaris L.) plants were obtained from a range of cultivated soils covering 16 geographical sites in Jordan. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for nodA, nifH and g7nII genes and sequencing methods showed that the rhizobial isolates formed two main groups. The first, which makes up 80% of isolates, were identified as Rhizobium etli while the second group comprising the remaining 20% were related to Rhizobium tropici. These findings indicate that R. etli is the predominant rhizobium that nodulates common bean in Jordan. (C) 2004 Elsevier B.V. All rights reserved.

KW - N-2 fixation

KW - Phaseolus valgaris

KW - R. etli

KW - R. tropici

KW - PCR-RFLP

KW - genotyping

KW - PHASEOLUS-VULGARIS L

KW - GENETIC DIVERSITY

KW - NIFH GENES

KW - NODULES

KW - STRAINS

KW - TROPICI

KW - AFRICA

KW - TREES

KW - FIELD

U2 - 10.1016/j.apsoil.2004.01.003

DO - 10.1016/j.apsoil.2004.01.003

M3 - Article

SN - 0929-1393

VL - 26

SP - 193

EP - 200

JO - Applied Soil Ecology

JF - Applied Soil Ecology

IS - 3

ER -