Ribosomal small subunit sequence diversity of Scutellospora  within single spores and roots of bluebell from a woodland community.

J.P. Clapp; J.P.W. Young; A.H. Fitter

doi:10.1046/j.1365-294x.1999.00642.x

Ribosomal small subunit sequence diversity of Scutellospora within single spores and roots of bluebell from a woodland community.

J.P. Clapp, J.P.W. Young, A.H. Fitter

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire,UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceaespecific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales,with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.

Original language	English
Pages (from-to)	915-921
Number of pages	6
Journal	Molecular Ecology
Volume	8
Issue number	6
DOIs	https://doi.org/10.1046/j.1365-294x.1999.00642.x
Publication status	Published - Jun 1999

Bibliographical note

© 1999 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com

Keywords

18S
arbuscular mycorrhiza
diversity
Glomales
rRNA
Scutellospora
SSU
VANS1

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Cite this

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title = "Ribosomal small subunit sequence diversity of Scutellospora within single spores and roots of bluebell from a woodland community.",

abstract = "Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire,UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceaespecific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales,with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.",

keywords = "18S, arbuscular mycorrhiza, diversity, Glomales, rRNA, Scutellospora, SSU, VANS1",

author = "J.P. Clapp and J.P.W. Young and A.H. Fitter",

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AU - Clapp, J.P.

AU - Young, J.P.W.

AU - Fitter, A.H.

N1 - © 1999 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com

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N2 - Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire,UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceaespecific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales,with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.

AB - Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire,UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceaespecific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales,with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.

KW - 18S

KW - arbuscular mycorrhiza

KW - diversity

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KW - rRNA

KW - Scutellospora

KW - SSU

KW - VANS1

U2 - 10.1046/j.1365-294x.1999.00642.x

DO - 10.1046/j.1365-294x.1999.00642.x

M3 - Article

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