RsrA, an anti-sigma factor regulated by redox change

J G Kang; M S Paget; Y J Seok; M Y Hahn; J B Bae; J S Hahn; C Kleanthous; M J Buttner; J H Roe; Colin Kleanthous

doi:10.1093/emboj/18.15.4292

RsrA, an anti-sigma factor regulated by redox change

J G Kang, M S Paget, Y J Seok, M Y Hahn, J B Bae, J S Hahn, C Kleanthous, M J Buttner, J H Roe, Colin Kleanthous

Research output: Contribution to journal › Article › peer-review

Abstract

SigR (sigma(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, sigma(R)-specific anti-sigma factor, RsrA, which binds sigma(R) and inhibits sigma(R)-directed transcription in vitro only under reducing conditions. Exposure to H(2)O(2) or to the thiol-specific oxidant diamide caused the dissociation of the sigma(R)-RsrA complex, thereby allowing sigma(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that sigma(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.

Original language	English
Pages (from-to)	4292-8
Number of pages	7
Journal	EMBO Journal
Volume	18
Issue number	15
DOIs	https://doi.org/10.1093/emboj/18.15.4292
Publication status	Published - 1999

Keywords

Amino Acid Sequence
Bacterial Proteins
Disulfides
Mass Spectrometry
Molecular Sequence Data
Oxidation-Reduction
Sequence Homology, Amino Acid
Sigma Factor
Substrate Specificity
Surface Plasmon Resonance
Thioredoxins
Transcription Factors
Transcription, Genetic

Access to Document

10.1093/emboj/18.15.4292

Cite this

@article{5ae5e03a0a004cb2b55e8827a534c811,

title = "RsrA, an anti-sigma factor regulated by redox change",

abstract = "SigR (sigma(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, sigma(R)-specific anti-sigma factor, RsrA, which binds sigma(R) and inhibits sigma(R)-directed transcription in vitro only under reducing conditions. Exposure to H(2)O(2) or to the thiol-specific oxidant diamide caused the dissociation of the sigma(R)-RsrA complex, thereby allowing sigma(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that sigma(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.",

keywords = "Amino Acid Sequence, Bacterial Proteins, Disulfides, Mass Spectrometry, Molecular Sequence Data, Oxidation-Reduction, Sequence Homology, Amino Acid, Sigma Factor, Substrate Specificity, Surface Plasmon Resonance, Thioredoxins, Transcription Factors, Transcription, Genetic",

author = "Kang, {J G} and Paget, {M S} and Seok, {Y J} and Hahn, {M Y} and Bae, {J B} and Hahn, {J S} and C Kleanthous and Buttner, {M J} and Roe, {J H} and Colin Kleanthous",

year = "1999",

doi = "10.1093/emboj/18.15.4292",

language = "English",

volume = "18",

pages = "4292--8",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Springer",

number = "15",

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TY - JOUR

T1 - RsrA, an anti-sigma factor regulated by redox change

AU - Kang, J G

AU - Paget, M S

AU - Seok, Y J

AU - Hahn, M Y

AU - Bae, J B

AU - Hahn, J S

AU - Kleanthous, C

AU - Buttner, M J

AU - Roe, J H

AU - Kleanthous, Colin

PY - 1999

Y1 - 1999

N2 - SigR (sigma(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, sigma(R)-specific anti-sigma factor, RsrA, which binds sigma(R) and inhibits sigma(R)-directed transcription in vitro only under reducing conditions. Exposure to H(2)O(2) or to the thiol-specific oxidant diamide caused the dissociation of the sigma(R)-RsrA complex, thereby allowing sigma(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that sigma(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.

AB - SigR (sigma(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, sigma(R)-specific anti-sigma factor, RsrA, which binds sigma(R) and inhibits sigma(R)-directed transcription in vitro only under reducing conditions. Exposure to H(2)O(2) or to the thiol-specific oxidant diamide caused the dissociation of the sigma(R)-RsrA complex, thereby allowing sigma(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that sigma(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.

KW - Amino Acid Sequence

KW - Bacterial Proteins

KW - Disulfides

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Oxidation-Reduction

KW - Sequence Homology, Amino Acid

KW - Sigma Factor

KW - Substrate Specificity

KW - Surface Plasmon Resonance

KW - Thioredoxins

KW - Transcription Factors

KW - Transcription, Genetic

U2 - 10.1093/emboj/18.15.4292

DO - 10.1093/emboj/18.15.4292

M3 - Article

C2 - 10428967

SN - 0261-4189

VL - 18

SP - 4292

EP - 4298

JO - EMBO Journal

JF - EMBO Journal

IS - 15

ER -