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S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells

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JournalNucleic Acids Research
DateAccepted/In press - 2 Jul 2016
DateE-pub ahead of print - 12 Jul 2016
DatePublished (current) - 16 Nov 2016
Issue number20
Number of pages14
Pages (from-to)9942-9955
Early online date12/07/16
Original languageEnglish


MicroRNAs (miRNAs) are short non-coding RNAs that silence mRNAs. They are generated following transcription and cleavage by the DROSHA/DGCR8 and DICER/TRBP/PACT complexes. Although it is known that components of the miRNA biogenesis machinery can be phosphorylated, it remains poorly understood how these events become engaged during physiological cellular activation. We demonstrate that S6 kinases can phosphorylate the extended C-terminal domain of TRBP and interact with TRBP in situ in primary cells. TRBP serines 283/286 are essential for S6K-mediated TRBP phosphorylation, optimal expression of TRBP, and the S6K-TRBP interaction in human primary cells. We demonstrate the functional relevance of this interaction in primary human dermal lymphatic endothelial cells (HDLECs). Angiopoietin-1 (ANG1) can augment miRNA biogenesis in HDLECs through enhancing TRBP phosphorylation and expression in an S6K2-dependent manner. We propose that the S6K2/TRBP node controls miRNA biogenesis in HDLECs and provides a molecular link between the mTOR pathway and the miRNA biogenesis machinery.

Bibliographical note

Funding Information:
We would like to thank Banushan Balansethupathy for technical contributions. Wellcome Trust [097829; through the Centre for Chronic Diseases and Disorders at the University of York]; Medical Research Council [MR/L008505/1]; Yorkshire Cancer Research [TPP053 to D.L.]; BBSRC [BB/I007571/2 to T.V.S.]. 'Combating Infectious Disease: Computational Approaches in Translational Science' Wellcome Trust PhD programme [WT095024MA to A.P.H.]. Funding for open access charge: The University of York Open Access Fund through agreement with the Medical Research Council and The Wellcome Trust who funded the work. Conflict of interest statement. None declared.

Publisher Copyright:
© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Copyright 2017 Elsevier B.V., All rights reserved.

    Research areas

  • Angiopoietin-1/pharmacology, Cell Line, Cells, Cultured, Endothelial Cells/drug effects, Gene Expression Regulation/drug effects, Humans, MicroRNAs/genetics, Phosphorylation, Protein Interaction Domains and Motifs, RNA-Binding Proteins/chemistry, Ribosomal Protein S6 Kinases/metabolism

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