SABRE-enhanced real-time pure shift NMR spectroscopy

TY - JOUR

T1 - SABRE-enhanced real-time pure shift NMR spectroscopy

AU - Taylor, Daniel A

AU - Natrajan, Louise S

AU - Nilsson, Mathias

AU - Adams, Ralph W

N1 - © 2021 John Wiley & Sons, Ltd.

PY - 2021/11/14

Y1 - 2021/11/14

N2 - Pure shift nuclear magnetic resonance (NMR) methods suppress the effect of homonuclear scalar couplings to produce NMR spectra consisting solely of a single signal for each chemically distinct site. They are increasingly relied upon for analysis of complex molecules and mixtures as they overcome the extensive signal overlap that complicates proton NMR spectra of all but the simplest species. Current broadband pure shift methodologies for 1D proton spectra suffer from reduced sensitivity compared with their conventional counterparts and typically require a large amount of instrument time for low concentration samples. In this study, we demonstrate how the sensitivity limitation may be overcome by transiently increasing the bulk polarization using signal amplification by reversible exchange (SABRE) hyperpolarization. We utilize para-enriched dihydrogen to enhance the pure shift NMR resonances of pyridine by up to a factor of 60 in a single-scan experiment and extend this to propose a method to unambiguously determine mixture components based on the enhancement of their pure shift NMR signals.

AB - Pure shift nuclear magnetic resonance (NMR) methods suppress the effect of homonuclear scalar couplings to produce NMR spectra consisting solely of a single signal for each chemically distinct site. They are increasingly relied upon for analysis of complex molecules and mixtures as they overcome the extensive signal overlap that complicates proton NMR spectra of all but the simplest species. Current broadband pure shift methodologies for 1D proton spectra suffer from reduced sensitivity compared with their conventional counterparts and typically require a large amount of instrument time for low concentration samples. In this study, we demonstrate how the sensitivity limitation may be overcome by transiently increasing the bulk polarization using signal amplification by reversible exchange (SABRE) hyperpolarization. We utilize para-enriched dihydrogen to enhance the pure shift NMR resonances of pyridine by up to a factor of 60 in a single-scan experiment and extend this to propose a method to unambiguously determine mixture components based on the enhancement of their pure shift NMR signals.

KW - Hydrogen

KW - Magnetic Resonance Imaging

KW - Magnetic Resonance Spectroscopy

KW - Protons

U2 - 10.1002/mrc.5206

DO - 10.1002/mrc.5206

M3 - Article

C2 - 34405451

SN - 0749-1581

VL - 59

SP - 1244

EP - 1252

JO - MAGNETIC RESONANCE IN CHEMISTRY

JF - MAGNETIC RESONANCE IN CHEMISTRY

IS - 12

ER -