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Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles

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Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles. / Orfanos, Zacharias; Leonard, Kevin; Elliott, Chris; Katzemich, Anja; Bullard, Belinda; Sparrow, John.

In: Journal of Molecular Biology, Vol. 427, No. 12, 19.06.2015, p. 2151-2158.

Research output: Contribution to journalArticlepeer-review

Harvard

Orfanos, Z, Leonard, K, Elliott, C, Katzemich, A, Bullard, B & Sparrow, J 2015, 'Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles', Journal of Molecular Biology, vol. 427, no. 12, pp. 2151-2158. https://doi.org/10.1016/j.jmb.2015.04.003

APA

Orfanos, Z., Leonard, K., Elliott, C., Katzemich, A., Bullard, B., & Sparrow, J. (2015). Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles. Journal of Molecular Biology, 427(12), 2151-2158. https://doi.org/10.1016/j.jmb.2015.04.003

Vancouver

Orfanos Z, Leonard K, Elliott C, Katzemich A, Bullard B, Sparrow J. Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles. Journal of Molecular Biology. 2015 Jun 19;427(12):2151-2158. https://doi.org/10.1016/j.jmb.2015.04.003

Author

Orfanos, Zacharias ; Leonard, Kevin ; Elliott, Chris ; Katzemich, Anja ; Bullard, Belinda ; Sparrow, John. / Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles. In: Journal of Molecular Biology. 2015 ; Vol. 427, No. 12. pp. 2151-2158.

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@article{fc2ae3731d8a4ac48c9e326cbf9a28c5,
title = "Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles",
abstract = "The Drosophila indirect flight muscles (IFM) can be used as a model for the study of sarcomere assembly. Here we use a transgenic line with a green fluorescent protein (GFP) exon inserted into the Z-disc-proximal portion of sallimus (Sls), also known as Drosophila titin, to observe sarcomere assembly during IFM development. Firstly, we confirm that Sls-GFP can be used in the heterozygote state without an obvious phenotype in IFM and other muscles. We then use Sls-GFP in the IFM to show that sarcomeres grow individually and uniformly throughout the fibre, growing linearly in length and in diameter. Finally, we show that limiting the amounts of Sls in the IFM using RNAi leads to sarcomeres with smaller Z-discs in their core, whilst the thick/thin filament lattice can form peripherally without a Z-disc. Thick filament preparations from those muscles show that although the Z-disc-containing core has thick filaments of a regular length, filaments from the peripheral lattice are longer and asymmetrical around the bare zone. Therefore, the Z-disc and Sls are required for thick filament length specification but not for the assembly of the thin/thick filament lattice.",
author = "Zacharias Orfanos and Kevin Leonard and Chris Elliott and Anja Katzemich and Belinda Bullard and John Sparrow",
note = "Copyright {\textcopyright} 2015 Elsevier Ltd. All rights reserved.",
year = "2015",
month = jun,
day = "19",
doi = "10.1016/j.jmb.2015.04.003",
language = "English",
volume = "427",
pages = "2151--2158",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "12",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Sallimus and the dynamics of sarcomere assembly in Drosophila flight muscles

AU - Orfanos, Zacharias

AU - Leonard, Kevin

AU - Elliott, Chris

AU - Katzemich, Anja

AU - Bullard, Belinda

AU - Sparrow, John

N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/6/19

Y1 - 2015/6/19

N2 - The Drosophila indirect flight muscles (IFM) can be used as a model for the study of sarcomere assembly. Here we use a transgenic line with a green fluorescent protein (GFP) exon inserted into the Z-disc-proximal portion of sallimus (Sls), also known as Drosophila titin, to observe sarcomere assembly during IFM development. Firstly, we confirm that Sls-GFP can be used in the heterozygote state without an obvious phenotype in IFM and other muscles. We then use Sls-GFP in the IFM to show that sarcomeres grow individually and uniformly throughout the fibre, growing linearly in length and in diameter. Finally, we show that limiting the amounts of Sls in the IFM using RNAi leads to sarcomeres with smaller Z-discs in their core, whilst the thick/thin filament lattice can form peripherally without a Z-disc. Thick filament preparations from those muscles show that although the Z-disc-containing core has thick filaments of a regular length, filaments from the peripheral lattice are longer and asymmetrical around the bare zone. Therefore, the Z-disc and Sls are required for thick filament length specification but not for the assembly of the thin/thick filament lattice.

AB - The Drosophila indirect flight muscles (IFM) can be used as a model for the study of sarcomere assembly. Here we use a transgenic line with a green fluorescent protein (GFP) exon inserted into the Z-disc-proximal portion of sallimus (Sls), also known as Drosophila titin, to observe sarcomere assembly during IFM development. Firstly, we confirm that Sls-GFP can be used in the heterozygote state without an obvious phenotype in IFM and other muscles. We then use Sls-GFP in the IFM to show that sarcomeres grow individually and uniformly throughout the fibre, growing linearly in length and in diameter. Finally, we show that limiting the amounts of Sls in the IFM using RNAi leads to sarcomeres with smaller Z-discs in their core, whilst the thick/thin filament lattice can form peripherally without a Z-disc. Thick filament preparations from those muscles show that although the Z-disc-containing core has thick filaments of a regular length, filaments from the peripheral lattice are longer and asymmetrical around the bare zone. Therefore, the Z-disc and Sls are required for thick filament length specification but not for the assembly of the thin/thick filament lattice.

U2 - 10.1016/j.jmb.2015.04.003

DO - 10.1016/j.jmb.2015.04.003

M3 - Article

C2 - 25868382

VL - 427

SP - 2151

EP - 2158

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 12

ER -