Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

Luca Longanesi; Florent P. Bouxin; Jiajun Fan; Hadiza Auta; Richard Gammons; Felix Abeln; Vitaliy L. Budarin; James H. Clark; Christopher J. Chuck

doi:10.1021/acs.iecr.0c03463

Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

Luca Longanesi, Florent P. Bouxin, Jiajun Fan, Hadiza Auta, Richard Gammons, Felix Abeln, Vitaliy L. Budarin, James H. Clark, Christopher J. Chuck^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The cultivation of oleaginous yeast on second-generation feedstocks is an attractive alternative for edible lipid production. Despite many studies in this area addressing single aspects of this bioprocess, one of the major bottlenecks is the integration and optimization of the multiple unit operations while demonstrating this on a larger scale. In this investigation, a microwave-assisted hydrothermal process was developed to solubilize distillers' dried grains with solubles (DDGS). The optimal MW process was run semicontinuously and produced a fermentable oligosaccharide-rich stream with negligible furan-based content. An overliming step and reverse-osmosis stage were demonstrated, increasing the solubilized carbohydrate content to over 60 g/L, suitably concentrated for further bioprocessing. The oleaginous yeast, Metschnikowia pulcherrima, was used to ferment the material and was demonstrated to metabolize up to 75% of the oligosaccharide pool and produced 41% lipid content at a concentration of 14 g/L in a 27 day semicontinuous process.

Original language	English
Pages (from-to)	19803-19816
Number of pages	14
Journal	Industrial and Engineering Chemistry Research
Volume	59
Issue number	44
Early online date	26 Oct 2020
DOIs	https://doi.org/10.1021/acs.iecr.0c03463
Publication status	Published - 4 Nov 2020

Access to Document

10.1021/acs.iecr.0c03463

Cite this

@article{a973ee889b5a418fbb935ee203edc063,

title = "Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes",

abstract = "The cultivation of oleaginous yeast on second-generation feedstocks is an attractive alternative for edible lipid production. Despite many studies in this area addressing single aspects of this bioprocess, one of the major bottlenecks is the integration and optimization of the multiple unit operations while demonstrating this on a larger scale. In this investigation, a microwave-assisted hydrothermal process was developed to solubilize distillers' dried grains with solubles (DDGS). The optimal MW process was run semicontinuously and produced a fermentable oligosaccharide-rich stream with negligible furan-based content. An overliming step and reverse-osmosis stage were demonstrated, increasing the solubilized carbohydrate content to over 60 g/L, suitably concentrated for further bioprocessing. The oleaginous yeast, Metschnikowia pulcherrima, was used to ferment the material and was demonstrated to metabolize up to 75% of the oligosaccharide pool and produced 41% lipid content at a concentration of 14 g/L in a 27 day semicontinuous process. ",

author = "Luca Longanesi and Bouxin, {Florent P.} and Jiajun Fan and Hadiza Auta and Richard Gammons and Felix Abeln and Budarin, {Vitaliy L.} and Clark, {James H.} and Chuck, {Christopher J.}",

year = "2020",

month = nov,

day = "4",

doi = "10.1021/acs.iecr.0c03463",

language = "English",

volume = "59",

pages = "19803--19816",

journal = "Industrial and Engineering Chemistry Research",

issn = "0888-5885",

publisher = "American Chemical Society",

number = "44",

}

TY - JOUR

T1 - Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

AU - Longanesi, Luca

AU - Bouxin, Florent P.

AU - Fan, Jiajun

AU - Auta, Hadiza

AU - Gammons, Richard

AU - Abeln, Felix

AU - Budarin, Vitaliy L.

AU - Clark, James H.

AU - Chuck, Christopher J.

PY - 2020/11/4

Y1 - 2020/11/4

N2 - The cultivation of oleaginous yeast on second-generation feedstocks is an attractive alternative for edible lipid production. Despite many studies in this area addressing single aspects of this bioprocess, one of the major bottlenecks is the integration and optimization of the multiple unit operations while demonstrating this on a larger scale. In this investigation, a microwave-assisted hydrothermal process was developed to solubilize distillers' dried grains with solubles (DDGS). The optimal MW process was run semicontinuously and produced a fermentable oligosaccharide-rich stream with negligible furan-based content. An overliming step and reverse-osmosis stage were demonstrated, increasing the solubilized carbohydrate content to over 60 g/L, suitably concentrated for further bioprocessing. The oleaginous yeast, Metschnikowia pulcherrima, was used to ferment the material and was demonstrated to metabolize up to 75% of the oligosaccharide pool and produced 41% lipid content at a concentration of 14 g/L in a 27 day semicontinuous process.

AB - The cultivation of oleaginous yeast on second-generation feedstocks is an attractive alternative for edible lipid production. Despite many studies in this area addressing single aspects of this bioprocess, one of the major bottlenecks is the integration and optimization of the multiple unit operations while demonstrating this on a larger scale. In this investigation, a microwave-assisted hydrothermal process was developed to solubilize distillers' dried grains with solubles (DDGS). The optimal MW process was run semicontinuously and produced a fermentable oligosaccharide-rich stream with negligible furan-based content. An overliming step and reverse-osmosis stage were demonstrated, increasing the solubilized carbohydrate content to over 60 g/L, suitably concentrated for further bioprocessing. The oleaginous yeast, Metschnikowia pulcherrima, was used to ferment the material and was demonstrated to metabolize up to 75% of the oligosaccharide pool and produced 41% lipid content at a concentration of 14 g/L in a 27 day semicontinuous process.

UR - http://www.scopus.com/inward/record.url?scp=85095814709&partnerID=8YFLogxK

U2 - 10.1021/acs.iecr.0c03463

DO - 10.1021/acs.iecr.0c03463

M3 - Article

AN - SCOPUS:85095814709

SN - 0888-5885

VL - 59

SP - 19803

EP - 19816

JO - Industrial and Engineering Chemistry Research

JF - Industrial and Engineering Chemistry Research

IS - 44

ER -

Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

Abstract

Access to Document

Other files and links

Cite this