Second messengers of octopamine receptors in the snail Lymnaea

S Pitt; A Vehovszky; H Szabo; C J H Elliott

Second messengers of octopamine receptors in the snail Lymnaea

S Pitt, A Vehovszky, H Szabo, C J H Elliott

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We describe octopamine responses of 3 large buccal neurons of Lymnaea and test the hypothesis that these are cAMP-dependent.

The B1 neuron is excited by octopamine and the depolarisation is significantly enlarged (P < 0.05) by application of the blocker of cAMP breakdown, 3-isobutyl-1-methylxanthine (IBMX). The B1 neuron is also depolarised by forskolin, an activator of adenylyl cyclase.

The B2 and B3 neurons are inhibited by octopamine, and the response is not affected by IBMX Both cells are excited by forskolin.

We conclude that the B I neuron response to octopamine is likely to be mediated by cAMP, while the B2 and B3 responses are cAMP-independent.

Original language	English
Pages (from-to)	177-183
Number of pages	7
Journal	Acta biologica hungarica
Volume	55
Issue number	1-4
Publication status	Published - 2004

Keywords

Lymnaea stagnalis
feeding
octopamine
receptor
second messenger
CHOLINERGIC INTERNEURONS
FEEDING SYSTEM
STAGNALIS
NEURONS
LOCUST
ACTIVATION
CYCLASE
CLONING
CYCLES
MUSCLE

Cite this

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title = "Second messengers of octopamine receptors in the snail Lymnaea",

abstract = "We describe octopamine responses of 3 large buccal neurons of Lymnaea and test the hypothesis that these are cAMP-dependent.The B1 neuron is excited by octopamine and the depolarisation is significantly enlarged (P < 0.05) by application of the blocker of cAMP breakdown, 3-isobutyl-1-methylxanthine (IBMX). The B1 neuron is also depolarised by forskolin, an activator of adenylyl cyclase.The B2 and B3 neurons are inhibited by octopamine, and the response is not affected by IBMX Both cells are excited by forskolin.We conclude that the B I neuron response to octopamine is likely to be mediated by cAMP, while the B2 and B3 responses are cAMP-independent.",

keywords = "Lymnaea stagnalis, feeding, octopamine, receptor, second messenger, CHOLINERGIC INTERNEURONS, FEEDING SYSTEM, STAGNALIS, NEURONS, LOCUST, ACTIVATION, CYCLASE, CLONING, CYCLES, MUSCLE",

author = "S Pitt and A Vehovszky and H Szabo and Elliott, {C J H}",

year = "2004",

language = "English",

volume = "55",

pages = "177--183",

journal = "Acta biologica hungarica",

issn = "0236-5383",

publisher = "Akademiai Kiado",

number = "1-4",

}

TY - JOUR

T1 - Second messengers of octopamine receptors in the snail Lymnaea

AU - Pitt, S

AU - Vehovszky, A

AU - Szabo, H

AU - Elliott, C J H

PY - 2004

Y1 - 2004

N2 - We describe octopamine responses of 3 large buccal neurons of Lymnaea and test the hypothesis that these are cAMP-dependent.The B1 neuron is excited by octopamine and the depolarisation is significantly enlarged (P < 0.05) by application of the blocker of cAMP breakdown, 3-isobutyl-1-methylxanthine (IBMX). The B1 neuron is also depolarised by forskolin, an activator of adenylyl cyclase.The B2 and B3 neurons are inhibited by octopamine, and the response is not affected by IBMX Both cells are excited by forskolin.We conclude that the B I neuron response to octopamine is likely to be mediated by cAMP, while the B2 and B3 responses are cAMP-independent.

AB - We describe octopamine responses of 3 large buccal neurons of Lymnaea and test the hypothesis that these are cAMP-dependent.The B1 neuron is excited by octopamine and the depolarisation is significantly enlarged (P < 0.05) by application of the blocker of cAMP breakdown, 3-isobutyl-1-methylxanthine (IBMX). The B1 neuron is also depolarised by forskolin, an activator of adenylyl cyclase.The B2 and B3 neurons are inhibited by octopamine, and the response is not affected by IBMX Both cells are excited by forskolin.We conclude that the B I neuron response to octopamine is likely to be mediated by cAMP, while the B2 and B3 responses are cAMP-independent.

KW - Lymnaea stagnalis

KW - feeding

KW - octopamine

KW - receptor

KW - second messenger

KW - CHOLINERGIC INTERNEURONS

KW - FEEDING SYSTEM

KW - STAGNALIS

KW - NEURONS

KW - LOCUST

KW - ACTIVATION

KW - CYCLASE

KW - CLONING

KW - CYCLES

KW - MUSCLE

M3 - Article

SN - 0236-5383

VL - 55

SP - 177

EP - 183

JO - Acta biologica hungarica

JF - Acta biologica hungarica

IS - 1-4

ER -