Abstract
Chemical modification of proteins is an important tool for probing natural systems, creating therapeutic conjugates and generating novel protein constructs. Site-selective reactions require exquisite control over both chemo- and regioselectivity, under ambient, aqueous conditions. There are now various methods for achieving selective modification of both natural and unnatural amino acids - each with merits and limitations - providing a toolkit that until 20 years ago was largely limited to reactions at nucleophilic cysteine and lysine residues. If applied in a biologically benign manner, this chemistry could form the basis of true Synthetic Biology.
Original language | English |
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Article number | 4740 |
Number of pages | 14 |
Journal | Nature Communications |
Volume | 5 |
DOIs | |
Publication status | Published - 5 Sept 2014 |