Selective chemical protein modification

Christopher D. Spicer, Benjamin G. Davis*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

Chemical modification of proteins is an important tool for probing natural systems, creating therapeutic conjugates and generating novel protein constructs. Site-selective reactions require exquisite control over both chemo- and regioselectivity, under ambient, aqueous conditions. There are now various methods for achieving selective modification of both natural and unnatural amino acids - each with merits and limitations - providing a toolkit that until 20 years ago was largely limited to reactions at nucleophilic cysteine and lysine residues. If applied in a biologically benign manner, this chemistry could form the basis of true Synthetic Biology.

Original languageEnglish
Article number4740
Number of pages14
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 5 Sept 2014

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