Selective Isotopic Unlabeling of Proteins Using Metabolic Precursors: Application to NMR Assignment of Intrinsically Disordered Proteins

TY - JOUR

T1 - Selective Isotopic Unlabeling of Proteins Using Metabolic Precursors

T2 - Application to NMR Assignment of Intrinsically Disordered Proteins

AU - Rasia, Rodolfo M.

AU - Brutscher, Bernhard

AU - Plevin, Michael J.

PY - 2012/3/19

Y1 - 2012/3/19

N2 - Selective isotopic unlabeling of proteins can provide important residue-type information as well as reduce congestion of NMR spectra. However, metabolic scrambling often complicates the final isotope-labeling pattern. Here, an array of metabolic precursors is used to perform robust, residue-specific unlabeling of proteins. The resulting isotopic-labeling patterns are predictable and nicely complement NMR experiments that differentiate residue types. This approach has widespread applications, but it is particularly relevant for proteins that lack sequence complexity or a defined tertiary structure.

AB - Selective isotopic unlabeling of proteins can provide important residue-type information as well as reduce congestion of NMR spectra. However, metabolic scrambling often complicates the final isotope-labeling pattern. Here, an array of metabolic precursors is used to perform robust, residue-specific unlabeling of proteins. The resulting isotopic-labeling patterns are predictable and nicely complement NMR experiments that differentiate residue types. This approach has widespread applications, but it is particularly relevant for proteins that lack sequence complexity or a defined tertiary structure.

UR - http://www.scopus.com/inward/record.url?scp=84858330492&partnerID=8YFLogxK

U2 - 10.1002/cbic.201100678

DO - 10.1002/cbic.201100678

M3 - Article

SN - 1439-4227

VL - 13

SP - 732

EP - 739

JO - Chembiochem

JF - Chembiochem

IS - 5

ER -