Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability

Gianpaolo Dagrada; Katia Rupel; Serena Zacchigna; Elena Tamborini; Silvana Pilotti; Adalberto Cavalleri; Loryn E. Fechner; Erik Laurini; David Kelham Smith; Silvia Brich; Sabrina Pricl

doi:10.1021/acs.molpharmaceut.8b00655

Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability

Gianpaolo Dagrada, Katia Rupel, Serena Zacchigna, Elena Tamborini, Silvana Pilotti, Adalberto Cavalleri, Loryn E. Fechner, Erik Laurini, David Kelham Smith, Silvia Brich, Sabrina Pricl

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Solitary fibrous tumors (SFTs) are rare soft tissue sarcomas that rely on several epithelial-mesenchymal transition (EMT) protein regulators for invasion/metastatic progression. Curcumin (CUR) has several pharmacological activities, including anticancer activity and the ability to suppress the EMT process. However, poor absorption, rapid metabolism, and side effects at high
doses limit the clinical applications of CUR. Here we present the results obtained by treating SFT cells with free CUR and three different CUR-loaded nanomicelles (NMs), each of which has its surface decorated with different ligands. All CUR-loaded NMs were more efficient in suppressing SFT cell viability and expression of EMT markers than CUR alone. Combined treatments with the pan-histone
deacetylase dual inhibitor SAHA revealed a differential ability in inhibiting EMT markers expression and SFT cell invasiveness, depending on the NM-ligand type. Finally, combinations of photodynamic therapy and CUR-loaded NM administrations resulted in almost complete SFT cell viability abrogation 24 h after laser irradiation.

Original language	English
Pages (from-to)	4689-4701
Number of pages	13
Journal	MOLECULAR PHARMACEUTICS
Volume	15
Early online date	4 Sept 2018
DOIs	https://doi.org/10.1021/acs.molpharmaceut.8b00655
Publication status	Published - Oct 2018

Bibliographical note

© 2018 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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10.1021/acs.molpharmaceut.8b00655

acs.molpharmaceut.8b00655
© 2018 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
Accepted author manuscript, 19.4 MB

Cite this

Dagrada, G., Rupel, K., Zacchigna, S., Tamborini, E., Pilotti, S., Cavalleri, A., Fechner, L. E., Laurini, E., Smith, D. K., Brich, S., & Pricl, S. (2018). Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability. MOLECULAR PHARMACEUTICS, 15, 4689-4701. https://doi.org/10.1021/acs.molpharmaceut.8b00655

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abstract = "Solitary fibrous tumors (SFTs) are rare soft tissue sarcomas that rely on several epithelial-mesenchymal transition (EMT) protein regulators for invasion/metastatic progression. Curcumin (CUR) has several pharmacological activities, including anticancer activity and the ability to suppress the EMT process. However, poor absorption, rapid metabolism, and side effects at highdoses limit the clinical applications of CUR. Here we present the results obtained by treating SFT cells with free CUR and three different CUR-loaded nanomicelles (NMs), each of which has its surface decorated with different ligands. All CUR-loaded NMs were more efficient in suppressing SFT cell viability and expression of EMT markers than CUR alone. Combined treatments with the pan-histonedeacetylase dual inhibitor SAHA revealed a differential ability in inhibiting EMT markers expression and SFT cell invasiveness, depending on the NM-ligand type. Finally, combinations of photodynamic therapy and CUR-loaded NM administrations resulted in almost complete SFT cell viability abrogation 24 h after laser irradiation.",

author = "Gianpaolo Dagrada and Katia Rupel and Serena Zacchigna and Elena Tamborini and Silvana Pilotti and Adalberto Cavalleri and Fechner, {Loryn E.} and Erik Laurini and Smith, {David Kelham} and Silvia Brich and Sabrina Pricl",

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Dagrada, G, Rupel, K, Zacchigna, S, Tamborini, E, Pilotti, S, Cavalleri, A, Fechner, LE, Laurini, E, Smith, DK, Brich, S & Pricl, S 2018, 'Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability', MOLECULAR PHARMACEUTICS, vol. 15, pp. 4689-4701. https://doi.org/10.1021/acs.molpharmaceut.8b00655

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AU - Dagrada, Gianpaolo

AU - Rupel, Katia

AU - Zacchigna, Serena

AU - Tamborini, Elena

AU - Pilotti, Silvana

AU - Cavalleri, Adalberto

AU - Fechner, Loryn E.

AU - Laurini, Erik

AU - Smith, David Kelham

AU - Brich, Silvia

AU - Pricl, Sabrina

N1 - © 2018 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2018/10

Y1 - 2018/10

N2 - Solitary fibrous tumors (SFTs) are rare soft tissue sarcomas that rely on several epithelial-mesenchymal transition (EMT) protein regulators for invasion/metastatic progression. Curcumin (CUR) has several pharmacological activities, including anticancer activity and the ability to suppress the EMT process. However, poor absorption, rapid metabolism, and side effects at highdoses limit the clinical applications of CUR. Here we present the results obtained by treating SFT cells with free CUR and three different CUR-loaded nanomicelles (NMs), each of which has its surface decorated with different ligands. All CUR-loaded NMs were more efficient in suppressing SFT cell viability and expression of EMT markers than CUR alone. Combined treatments with the pan-histonedeacetylase dual inhibitor SAHA revealed a differential ability in inhibiting EMT markers expression and SFT cell invasiveness, depending on the NM-ligand type. Finally, combinations of photodynamic therapy and CUR-loaded NM administrations resulted in almost complete SFT cell viability abrogation 24 h after laser irradiation.

AB - Solitary fibrous tumors (SFTs) are rare soft tissue sarcomas that rely on several epithelial-mesenchymal transition (EMT) protein regulators for invasion/metastatic progression. Curcumin (CUR) has several pharmacological activities, including anticancer activity and the ability to suppress the EMT process. However, poor absorption, rapid metabolism, and side effects at highdoses limit the clinical applications of CUR. Here we present the results obtained by treating SFT cells with free CUR and three different CUR-loaded nanomicelles (NMs), each of which has its surface decorated with different ligands. All CUR-loaded NMs were more efficient in suppressing SFT cell viability and expression of EMT markers than CUR alone. Combined treatments with the pan-histonedeacetylase dual inhibitor SAHA revealed a differential ability in inhibiting EMT markers expression and SFT cell invasiveness, depending on the NM-ligand type. Finally, combinations of photodynamic therapy and CUR-loaded NM administrations resulted in almost complete SFT cell viability abrogation 24 h after laser irradiation.

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DO - 10.1021/acs.molpharmaceut.8b00655

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