Sequence diversity of the plasmid replication gene repC in the Rhizobiaceae

K M Palmer; S L Turner; J P W Young

doi:10.1006/plas.2000.1488

Sequence diversity of the plasmid replication gene repC in the Rhizobiaceae

K M Palmer, S L Turner, J P W Young

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The repABC operon is essential for stable maintenance of some Rhizobiaceae plasmids and of pTAV320 from Paracoccus versutus. These plasmids are the largest described family of homologous, yet compatible replicons. The repC gene is essential for plasmid replication, and previous work identified four distinct sequence groups (repC1, repC2, repC3 and repC4) that appear to define different compatibility classes. Probes for these different groups were used to characterize plasmids in Rhizobium leguminosarum population studies and three new repC sequence groups, repC5, repC6, and repC7 were identified. The general repC primers were modified to amplify a wider range of repC sequences and repC sequences were indentified in Sinorhizobium and Mesorhizobium type strains. We also showed that the repC3 group-specific primers described previously do not amplify all repC3 sequences and developed a new repC3 amplification strategy. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	209-219
Number of pages	11
Journal	Plasmid
Volume	44
Issue number	3
DOIs	https://doi.org/10.1006/plas.2000.1488
Publication status	Published - Nov 2000

Keywords

BACTERIAL PLASMIDS
CLONING VECTORS
CRYPTIC PLASMID
REGION
IDENTIFICATION
LEGUMINOSARUM
REPLICON
HOMOLOGY
GENOME
SHOW

Access to Document

10.1006/plas.2000.1488

Cite this

@article{c458769a1c054c76820c31f13ba1d400,

title = "Sequence diversity of the plasmid replication gene repC in the Rhizobiaceae",

abstract = "The repABC operon is essential for stable maintenance of some Rhizobiaceae plasmids and of pTAV320 from Paracoccus versutus. These plasmids are the largest described family of homologous, yet compatible replicons. The repC gene is essential for plasmid replication, and previous work identified four distinct sequence groups (repC1, repC2, repC3 and repC4) that appear to define different compatibility classes. Probes for these different groups were used to characterize plasmids in Rhizobium leguminosarum population studies and three new repC sequence groups, repC5, repC6, and repC7 were identified. The general repC primers were modified to amplify a wider range of repC sequences and repC sequences were indentified in Sinorhizobium and Mesorhizobium type strains. We also showed that the repC3 group-specific primers described previously do not amplify all repC3 sequences and developed a new repC3 amplification strategy. (C) 2000 Academic Press.",

keywords = "BACTERIAL PLASMIDS, CLONING VECTORS, CRYPTIC PLASMID, REGION, IDENTIFICATION, LEGUMINOSARUM, REPLICON, HOMOLOGY, GENOME, SHOW",

author = "Palmer, {K M} and Turner, {S L} and Young, {J P W}",

year = "2000",

month = nov,

doi = "10.1006/plas.2000.1488",

language = "English",

volume = "44",

pages = "209--219",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press",

number = "3",

}

TY - JOUR

T1 - Sequence diversity of the plasmid replication gene repC in the Rhizobiaceae

AU - Palmer, K M

AU - Turner, S L

AU - Young, J P W

PY - 2000/11

Y1 - 2000/11

N2 - The repABC operon is essential for stable maintenance of some Rhizobiaceae plasmids and of pTAV320 from Paracoccus versutus. These plasmids are the largest described family of homologous, yet compatible replicons. The repC gene is essential for plasmid replication, and previous work identified four distinct sequence groups (repC1, repC2, repC3 and repC4) that appear to define different compatibility classes. Probes for these different groups were used to characterize plasmids in Rhizobium leguminosarum population studies and three new repC sequence groups, repC5, repC6, and repC7 were identified. The general repC primers were modified to amplify a wider range of repC sequences and repC sequences were indentified in Sinorhizobium and Mesorhizobium type strains. We also showed that the repC3 group-specific primers described previously do not amplify all repC3 sequences and developed a new repC3 amplification strategy. (C) 2000 Academic Press.

AB - The repABC operon is essential for stable maintenance of some Rhizobiaceae plasmids and of pTAV320 from Paracoccus versutus. These plasmids are the largest described family of homologous, yet compatible replicons. The repC gene is essential for plasmid replication, and previous work identified four distinct sequence groups (repC1, repC2, repC3 and repC4) that appear to define different compatibility classes. Probes for these different groups were used to characterize plasmids in Rhizobium leguminosarum population studies and three new repC sequence groups, repC5, repC6, and repC7 were identified. The general repC primers were modified to amplify a wider range of repC sequences and repC sequences were indentified in Sinorhizobium and Mesorhizobium type strains. We also showed that the repC3 group-specific primers described previously do not amplify all repC3 sequences and developed a new repC3 amplification strategy. (C) 2000 Academic Press.

KW - BACTERIAL PLASMIDS

KW - CLONING VECTORS

KW - CRYPTIC PLASMID

KW - REGION

KW - IDENTIFICATION

KW - LEGUMINOSARUM

KW - REPLICON

KW - HOMOLOGY

KW - GENOME

KW - SHOW

U2 - 10.1006/plas.2000.1488

DO - 10.1006/plas.2000.1488

M3 - Article

SN - 0147-619X

VL - 44

SP - 209

EP - 219

JO - Plasmid

JF - Plasmid

IS - 3

ER -