Sequencing-based variant detection in the polyploid crop oilseed rape

TY - JOUR

T1 - Sequencing-based variant detection in the polyploid crop oilseed rape

AU - Wells, Rachel

AU - Trick, Martin

AU - Fraser, Fiona

AU - Soumpourou, Eleni

AU - Clissold, Leah

AU - Morgan, Colin

AU - Pauquet, Jérôme

AU - Bancroft, Ian

PY - 2013/8/6

Y1 - 2013/8/6

N2 - The detection and exploitation of genetic variation underpins crop improvement. However, the polyploid nature of the genomes of many of our most important crops represents a barrier, particularly for the analysis of variation within genes. To overcome this, we aimed to develop methodologies based on amplicon sequencing that involve the incorporation of barcoded amplification tags (BATs) into PCR products.

AB - The detection and exploitation of genetic variation underpins crop improvement. However, the polyploid nature of the genomes of many of our most important crops represents a barrier, particularly for the analysis of variation within genes. To overcome this, we aimed to develop methodologies based on amplicon sequencing that involve the incorporation of barcoded amplification tags (BATs) into PCR products.

KW - Brassica napus

KW - Crops, Agricultural

KW - Genetic Linkage

KW - Genetic Variation

KW - Genome, Plant

KW - Genotype

KW - Mutation

KW - Plant Proteins

KW - Polymorphism, Single Nucleotide

KW - Polyploidy

KW - Sequence Analysis, DNA

U2 - 10.1186/1471-2229-13-111

DO - 10.1186/1471-2229-13-111

M3 - Article

C2 - 23915099

SN - 1471-2229

VL - 13

JO - BMC Plant Biology

JF - BMC Plant Biology

IS - 1

M1 - 111

ER -