Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency

TY - JOUR

T1 - Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency

AU - Walters, Alison D

AU - Smith, Sarah E

AU - Chong, James P J

PY - 2011/4

Y1 - 2011/4

N2 - We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.

AB - We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.

KW - Genetic Vectors

KW - Genetics, Microbial

KW - Methanococcus

KW - Transformation, Bacterial

UR - http://www.scopus.com/inward/record.url?scp=79953286120&partnerID=8YFLogxK

U2 - 10.1128/AEM.02919-10

DO - 10.1128/AEM.02919-10

M3 - Article

C2 - 21296937

SN - 0099-2240

VL - 77

SP - 2549

EP - 2551

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -