Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency

Research output: Contribution to journalArticlepeer-review


We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.
Original languageEnglish
Pages (from-to)2549-51
Number of pages3
JournalApplied and Environmental Microbiology
Issue number7
Early online date4 Feb 2011
Publication statusPublished - Apr 2011


  • Genetic Vectors
  • Genetics, Microbial
  • Methanococcus
  • Transformation, Bacterial

Cite this