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Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency

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JournalApplied and Environmental Microbiology
DateE-pub ahead of print - 4 Feb 2011
DatePublished (current) - Apr 2011
Issue number7
Volume77
Number of pages3
Pages (from-to)2549-51
Early online date4/02/11
Original languageEnglish

Abstract

We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.

    Research areas

  • Genetic Vectors, Genetics, Microbial, Methanococcus, Transformation, Bacterial

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