Simultaneous two-color imaging in digital holographic microscopy

Nicola Ellen Farthing; Rachel Christina Findlay; Jan F. Jikeli; Pegine Bavonne Walrad; Martin Alan Bees; Laurence George Wilson

doi:10.1364/OE.25.028489

Simultaneous two-color imaging in digital holographic microscopy

Nicola Ellen Farthing, Rachel Christina Findlay, Jan F. Jikeli, Pegine Bavonne Walrad, Martin Alan Bees, Laurence George Wilson

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in close
proximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.

Original language	English
Pages (from-to)	28489-28500
Number of pages	12
Journal	Optics Express
Volume	25
Issue number	23
DOIs	https://doi.org/10.1364/OE.25.028489
Publication status	Published - 13 Nov 2017

Bibliographical note

This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

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This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details
Accepted author manuscript, 686 KB

Two-colour holography I
Wilson, L. G. (Creator), University of York, 15 Aug 2017
DOI: 10.15124/5d61ce08-7ec5-4981-9980-fd4cc8a1b578
Dataset

Cite this

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abstract = "We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.",

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AU - Farthing, Nicola Ellen

AU - Findlay, Rachel Christina

AU - Jikeli, Jan F.

AU - Walrad, Pegine Bavonne

AU - Bees, Martin Alan

AU - Wilson, Laurence George

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N2 - We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.

AB - We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.

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DO - 10.1364/OE.25.028489

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Simultaneous two-color imaging in digital holographic microscopy

Abstract

Bibliographical note

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Datasets

Two-colour holography I

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