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Simultaneous two-color imaging in digital holographic microscopy

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Simultaneous two-color imaging in digital holographic microscopy. / Farthing, Nicola Ellen; Findlay, Rachel Christina; Jikeli, Jan F.; Walrad, Pegine Bavonne; Bees, Martin Alan; Wilson, Laurence George.

In: Optics Express, Vol. 25, No. 23, 13.11.2017, p. 28489-28500.

Research output: Contribution to journalArticle

Harvard

Farthing, NE, Findlay, RC, Jikeli, JF, Walrad, PB, Bees, MA & Wilson, LG 2017, 'Simultaneous two-color imaging in digital holographic microscopy', Optics Express, vol. 25, no. 23, pp. 28489-28500. https://doi.org/10.1364/OE.25.028489

APA

Farthing, N. E., Findlay, R. C., Jikeli, J. F., Walrad, P. B., Bees, M. A., & Wilson, L. G. (2017). Simultaneous two-color imaging in digital holographic microscopy. Optics Express, 25(23), 28489-28500. https://doi.org/10.1364/OE.25.028489

Vancouver

Farthing NE, Findlay RC, Jikeli JF, Walrad PB, Bees MA, Wilson LG. Simultaneous two-color imaging in digital holographic microscopy. Optics Express. 2017 Nov 13;25(23):28489-28500. https://doi.org/10.1364/OE.25.028489

Author

Farthing, Nicola Ellen ; Findlay, Rachel Christina ; Jikeli, Jan F. ; Walrad, Pegine Bavonne ; Bees, Martin Alan ; Wilson, Laurence George. / Simultaneous two-color imaging in digital holographic microscopy. In: Optics Express. 2017 ; Vol. 25, No. 23. pp. 28489-28500.

Bibtex - Download

@article{3bc3998011a34ff78c27a5dc39d3a3d6,
title = "Simultaneous two-color imaging in digital holographic microscopy",
abstract = "We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.",
author = "Farthing, {Nicola Ellen} and Findlay, {Rachel Christina} and Jikeli, {Jan F.} and Walrad, {Pegine Bavonne} and Bees, {Martin Alan} and Wilson, {Laurence George}",
note = "This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details",
year = "2017",
month = "11",
day = "13",
doi = "10.1364/OE.25.028489",
language = "English",
volume = "25",
pages = "28489--28500",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "23",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Simultaneous two-color imaging in digital holographic microscopy

AU - Farthing, Nicola Ellen

AU - Findlay, Rachel Christina

AU - Jikeli, Jan F.

AU - Walrad, Pegine Bavonne

AU - Bees, Martin Alan

AU - Wilson, Laurence George

N1 - This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

PY - 2017/11/13

Y1 - 2017/11/13

N2 - We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.

AB - We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly scattering objects in closeproximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a single-cell eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.

U2 - 10.1364/OE.25.028489

DO - 10.1364/OE.25.028489

M3 - Article

VL - 25

SP - 28489

EP - 28500

JO - Optics Express

T2 - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 23

ER -