Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide

Charlotte E. Dalton; Steven D. Quinn; Aidan Rafferty; Michael J Morten; John M Gardiner; Steven W. Magennis

doi:10.1002/cphc.201600750

Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide

Charlotte E. Dalton, Steven D. Quinn, Aidan Rafferty, Michael J Morten, John M Gardiner, Steven W. Magennis

Research output: Contribution to journal › Article › peer-review

Abstract

The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS–protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.

Original language	English
Pages (from-to)	3442-3446
Number of pages	5
Journal	ChemPhysChem
Volume	17
Issue number	21
Early online date	14 Sept 2016
DOIs	https://doi.org/10.1002/cphc.201600750
Publication status	Published - 4 Nov 2016

Bibliographical note

Keywords

carbohydrate
fluorescence
heparan sulfate
single-molecule studies
vesicle

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10.1002/cphc.201600750

Dalton_et_al-2016-ChemPhysChem
©2016 The Authors.
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Cite this

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AU - Quinn, Steven D.

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AU - Gardiner, John M

AU - Magennis, Steven W.

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AB - The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS–protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.

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Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide

Abstract

Bibliographical note

Keywords

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