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Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms

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Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms. / McQueen, Caitlin; Hughes, Gideon Llywelyn; Pownall, Mary Elizabeth.

In: Developmental Biology, Vol. 454, No. 1, 01.10.2019.

Research output: Contribution to journalArticle

Harvard

McQueen, C, Hughes, GL & Pownall, ME 2019, 'Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms', Developmental Biology, vol. 454, no. 1. https://doi.org/10.1016/j.ydbio.2019.06.001

APA

McQueen, C., Hughes, G. L., & Pownall, M. E. (2019). Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms. Developmental Biology, 454(1). https://doi.org/10.1016/j.ydbio.2019.06.001

Vancouver

McQueen C, Hughes GL, Pownall ME. Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms. Developmental Biology. 2019 Oct 1;454(1). https://doi.org/10.1016/j.ydbio.2019.06.001

Author

McQueen, Caitlin ; Hughes, Gideon Llywelyn ; Pownall, Mary Elizabeth. / Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms. In: Developmental Biology. 2019 ; Vol. 454, No. 1.

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@article{c70f8089c2d64a28a805e98562c593ae,
title = "Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms",
abstract = "Gene regulatory networks underpinning skeletal muscle determination and differentiation have been extensively investigated, providing molecular insights into how cell lineages are established during development. These studies have exclusively focused on the transcriptome downstream of RNA polymerase II (Pol II). RNA polymerase III (Pol III) drives the production of tRNAs and other small RNAs essential for the flow of genetic information from gene to protein and we have found that a specific isoform of a subunit unique to Pol III is expressed early in the myogenic lineage. This points to the possibility that additional regulatory networks exist to control the production of Pol III transcripts during skeletal muscle differentiation. We describe the differential expression of Polr3g and its alternate isoform Polr3gL during embryonic development and using a custom tRNA microarray, we demonstrate their distinct activity on the synthesis of tRNA isoacceptors. We show that Pol III dependent transcripts are dramatically down-regulated during the differentiation of skeletal muscle, as are mRNAs coding for Pol III associated proteins Brf1 and Brf2, while Polr3gL is up-regulated alongside contractile protein genes. Forcing Polr3g expression in this context results in a partial reversal of myogenic differentiation",
author = "Caitlin McQueen and Hughes, {Gideon Llywelyn} and Pownall, {Mary Elizabeth}",
note = "{\circledC} 2019 Elsevier Inc. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy.",
year = "2019",
month = "10",
day = "1",
doi = "10.1016/j.ydbio.2019.06.001",
language = "English",
volume = "454",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Skeletal muscle differentiation drives a dramatic downregulation of RNA polymerase III activity and differential expression of Polr3g isoforms

AU - McQueen, Caitlin

AU - Hughes, Gideon Llywelyn

AU - Pownall, Mary Elizabeth

N1 - © 2019 Elsevier Inc. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Gene regulatory networks underpinning skeletal muscle determination and differentiation have been extensively investigated, providing molecular insights into how cell lineages are established during development. These studies have exclusively focused on the transcriptome downstream of RNA polymerase II (Pol II). RNA polymerase III (Pol III) drives the production of tRNAs and other small RNAs essential for the flow of genetic information from gene to protein and we have found that a specific isoform of a subunit unique to Pol III is expressed early in the myogenic lineage. This points to the possibility that additional regulatory networks exist to control the production of Pol III transcripts during skeletal muscle differentiation. We describe the differential expression of Polr3g and its alternate isoform Polr3gL during embryonic development and using a custom tRNA microarray, we demonstrate their distinct activity on the synthesis of tRNA isoacceptors. We show that Pol III dependent transcripts are dramatically down-regulated during the differentiation of skeletal muscle, as are mRNAs coding for Pol III associated proteins Brf1 and Brf2, while Polr3gL is up-regulated alongside contractile protein genes. Forcing Polr3g expression in this context results in a partial reversal of myogenic differentiation

AB - Gene regulatory networks underpinning skeletal muscle determination and differentiation have been extensively investigated, providing molecular insights into how cell lineages are established during development. These studies have exclusively focused on the transcriptome downstream of RNA polymerase II (Pol II). RNA polymerase III (Pol III) drives the production of tRNAs and other small RNAs essential for the flow of genetic information from gene to protein and we have found that a specific isoform of a subunit unique to Pol III is expressed early in the myogenic lineage. This points to the possibility that additional regulatory networks exist to control the production of Pol III transcripts during skeletal muscle differentiation. We describe the differential expression of Polr3g and its alternate isoform Polr3gL during embryonic development and using a custom tRNA microarray, we demonstrate their distinct activity on the synthesis of tRNA isoacceptors. We show that Pol III dependent transcripts are dramatically down-regulated during the differentiation of skeletal muscle, as are mRNAs coding for Pol III associated proteins Brf1 and Brf2, while Polr3gL is up-regulated alongside contractile protein genes. Forcing Polr3g expression in this context results in a partial reversal of myogenic differentiation

UR - http://www.scopus.com/inward/record.url?scp=85067586382&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2019.06.001

DO - 10.1016/j.ydbio.2019.06.001

M3 - Article

VL - 454

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -