Soluble, folded and active subtilisin in a protic ionic liquid

Francesco Falcioni; Hazel R. Housden; Zhenlian Ling; Seishi Shimizu; Adam J. Walker; Neil C. Bruce

doi:10.1039/b916497a

Soluble, folded and active subtilisin in a protic ionic liquid

Francesco Falcioni, Hazel R. Housden, Zhenlian Ling, Seishi Shimizu, Adam J. Walker, Neil C. Bruce

Research output: Contribution to journal › Article › peer-review

Abstract

The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.

Original language	English
Pages (from-to)	749-751
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	5
DOIs	https://doi.org/10.1039/b916497a
Publication status	Published - 2010

Keywords

CATALYSIS
SOLVENTS

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10.1039/b916497a

http://pubs.rsc.org/en/Content/ArticleLanding/2010/CC/b916497a

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title = "Soluble, folded and active subtilisin in a protic ionic liquid",

abstract = "The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.",

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author = "Francesco Falcioni and Housden, {Hazel R.} and Zhenlian Ling and Seishi Shimizu and Walker, {Adam J.} and Bruce, {Neil C.}",

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AU - Falcioni, Francesco

AU - Housden, Hazel R.

AU - Ling, Zhenlian

AU - Shimizu, Seishi

AU - Walker, Adam J.

AU - Bruce, Neil C.

PY - 2010

Y1 - 2010

N2 - The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.

AB - The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.

KW - CATALYSIS

KW - SOLVENTS

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U2 - 10.1039/b916497a

DO - 10.1039/b916497a

M3 - Article

SN - 1359-7345

VL - 46

SP - 749

EP - 751

JO - Chemical Communications

JF - Chemical Communications

IS - 5

ER -

Soluble, folded and active subtilisin in a protic ionic liquid

Abstract

Keywords

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Other files and links

Advance optical waveguide biosensors for the detection of illicit drugs and explosives

Protein interactions in ionic media

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Soluble, folded and active subtilisin in a protic ionic liquid

Abstract

Keywords

Access to Document

Other files and links

Projects

Advance optical waveguide biosensors for the detection of illicit drugs and explosives

Protein interactions in ionic media

Cite this