Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

TY - JOUR

T1 - Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

AU - Buckley, Michael

AU - Collins, Matthew James

AU - Thomas-Oates, Jane

AU - Wilson, Julie C.

N1 - Copyright © 2009 John Wiley & Sons, Ltd.

PY - 2009/12/15

Y1 - 2009/12/15

N2 - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip (R)) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification. Copyright (C) 2009 John Wiley & Sons, Ltd.

AB - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip (R)) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification. Copyright (C) 2009 John Wiley & Sons, Ltd.

KW - MITOCHONDRIAL-DNA

KW - ANCIENT PROTEINS

KW - ANIMAL FEED

KW - MEAL

KW - OSTEOCALCIN

KW - PEPTIDES

KW - SEQUENCE

KW - PHYLOGENY

KW - EVOLUTION

KW - GELATIN

UR - http://www.scopus.com/inward/record.url?scp=71949102106&partnerID=8YFLogxK

U2 - 10.1002/rcm.4316

DO - 10.1002/rcm.4316

M3 - Article

SN - 0951-4198

VL - 23

SP - 3843

EP - 3854

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 23

ER -