By the same authors

From the same journal

Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalRapid Communications in Mass Spectrometry
DateE-pub ahead of print - 9 Nov 2009
DatePublished (current) - 15 Dec 2009
Issue number23
Volume23
Number of pages12
Pages (from-to)3843-3854
Early online date9/11/09
Original languageEnglish

Abstract

Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip (R)) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification. Copyright (C) 2009 John Wiley & Sons, Ltd.

Bibliographical note

Copyright © 2009 John Wiley & Sons, Ltd.

    Research areas

  • MITOCHONDRIAL-DNA, ANCIENT PROTEINS, ANIMAL FEED, MEAL, OSTEOCALCIN, PEPTIDES, SEQUENCE, PHYLOGENY, EVOLUTION, GELATIN

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations