SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects

Ian Berry; O. Dym; RM Esnouf; K Harlos; R. Meged; A. Perrakis; J. L. Sussman; TS Walter; Julie C. Wilson; Albrecht Messerschmidt

doi:10.1107/S090744490602943X

SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects

Ian Berry, O. Dym, RM Esnouf, K Harlos, R. Meged, A. Perrakis, J. L. Sussman, TS Walter, Julie C. Wilson, Albrecht Messerschmidt

Research output: Contribution to journal › Article › peer-review

Abstract

This paper reviews the developments in high- throughput and nanolitre- scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe ( SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.

Original language	English
Pages (from-to)	1137-1149
Number of pages	13
Journal	Acta crystallographica section d-Biological crystallography
Volume	62
Issue number	10
DOIs	https://doi.org/10.1107/S090744490602943X
Publication status	Published - Oct 2006

Keywords

THERMOTOGA-MARITIMA PROTEOME
STRUCTURAL GENOMICS
AUTOMATIC CLASSIFICATION
DRUG DISCOVERY
TRIALS
IMAGES
OPTIMIZATION
TRACKING
BIOLOGY

Access to Document

10.1107/S090744490602943X

Cite this

Berry, I., Dym, O., Esnouf, RM., Harlos, K., Meged, R., Perrakis, A., Sussman, J. L., Walter, TS., Wilson, J. C., & Messerschmidt, A. (2006). SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects. Acta crystallographica section d-Biological crystallography, 62(10), 1137-1149. https://doi.org/10.1107/S090744490602943X

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title = "SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects",

abstract = "This paper reviews the developments in high- throughput and nanolitre- scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe ( SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.",

keywords = "THERMOTOGA-MARITIMA PROTEOME, STRUCTURAL GENOMICS, AUTOMATIC CLASSIFICATION, DRUG DISCOVERY, TRIALS, IMAGES, OPTIMIZATION, TRACKING, BIOLOGY",

author = "Ian Berry and O. Dym and RM Esnouf and K Harlos and R. Meged and A. Perrakis and Sussman, {J. L.} and TS Walter and Wilson, {Julie C.} and Albrecht Messerschmidt",

year = "2006",

month = oct,

doi = "10.1107/S090744490602943X",

language = "English",

volume = "62",

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journal = "Acta crystallographica section d-Biological crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "10",

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Berry, I, Dym, O, Esnouf, RM, Harlos, K, Meged, R, Perrakis, A, Sussman, JL, Walter, TS, Wilson, JC & Messerschmidt, A 2006, 'SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects', Acta crystallographica section d-Biological crystallography, vol. 62, no. 10, pp. 1137-1149. https://doi.org/10.1107/S090744490602943X

SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects. / Berry, Ian; Dym, O.; Esnouf, RM et al.
In: Acta crystallographica section d-Biological crystallography, Vol. 62, No. 10, 10.2006, p. 1137-1149.

Research output: Contribution to journal › Article › peer-review

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KW - DRUG DISCOVERY

KW - TRIALS

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KW - OPTIMIZATION

KW - TRACKING

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