SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects

Ian Berry, O. Dym, RM Esnouf, K Harlos, R. Meged, A. Perrakis, J. L. Sussman, TS Walter, Julie C. Wilson, Albrecht Messerschmidt

Research output: Contribution to journalArticlepeer-review

Abstract

This paper reviews the developments in high- throughput and nanolitre- scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe ( SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.

Original languageEnglish
Pages (from-to)1137-1149
Number of pages13
JournalActa crystallographica section d-Biological crystallography
Volume62
Issue number10
DOIs
Publication statusPublished - Oct 2006

Keywords

  • THERMOTOGA-MARITIMA PROTEOME
  • STRUCTURAL GENOMICS
  • AUTOMATIC CLASSIFICATION
  • DRUG DISCOVERY
  • TRIALS
  • IMAGES
  • OPTIMIZATION
  • TRACKING
  • BIOLOGY

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