Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase

Fabián E Vaistij; Louise Jones; David C Baulcombe

doi:10.1105/tpc.010480

Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase

Fabián E Vaistij, Louise Jones, David C Baulcombe

Research output: Contribution to journal › Article › peer-review

Abstract

RNA silencing is a sequence-specific RNA degradation process that follows the recognition of double-stranded RNA. Here, we show that virus vectors carrying parts of a green fluorescent protein (GFP) transgene targeted RNA silencing in Nicotiana benthamiana and Arabidopsis against the entire GFP RNA. These results indicate that there was spreading of RNA targeting from the initiator region into the adjacent 5'and 3' regions of the target gene. Spreading was accompanied by methylation of the corresponding GFP DNA. It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2. These findings indicate that SDE1/SGS2 produces doublestranded RNA using the target RNA as a template. RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2.

Original language	English
Pages (from-to)	857-867
Number of pages	10
Journal	The Plant Cell
Volume	14
Issue number	4
DOIs	https://doi.org/10.1105/tpc.010480
Publication status	Published - Apr 2002

Keywords

Arabidopsis
Arabidopsis Proteins
DNA Methylation
Gene Silencing
Green Fluorescent Proteins
Luminescent Proteins
Oxidoreductases
Plant Proteins
Plants, Genetically Modified
RNA Replicase
RNA, Plant
RNA-Induced Silencing Complex
Ribonucleoproteins
Ribulose-Bisphosphate Carboxylase
Tobacco
Transcription, Genetic

Access to Document

10.1105/tpc.010480

http://dx.doi.org/10.1105/tpc.010480

Cite this

@article{f4e5b907bd2147f69fd1e5185e839d68,

title = "Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase",

abstract = "RNA silencing is a sequence-specific RNA degradation process that follows the recognition of double-stranded RNA. Here, we show that virus vectors carrying parts of a green fluorescent protein (GFP) transgene targeted RNA silencing in Nicotiana benthamiana and Arabidopsis against the entire GFP RNA. These results indicate that there was spreading of RNA targeting from the initiator region into the adjacent 5'and 3' regions of the target gene. Spreading was accompanied by methylation of the corresponding GFP DNA. It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2. These findings indicate that SDE1/SGS2 produces doublestranded RNA using the target RNA as a template. RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2.",

keywords = "Arabidopsis, Arabidopsis Proteins, DNA Methylation, Gene Silencing, Green Fluorescent Proteins, Luminescent Proteins, Oxidoreductases, Plant Proteins, Plants, Genetically Modified, RNA Replicase, RNA, Plant, RNA-Induced Silencing Complex, Ribonucleoproteins, Ribulose-Bisphosphate Carboxylase, Tobacco, Transcription, Genetic",

author = "Vaistij, {Fabi{\'a}n E} and Louise Jones and Baulcombe, {David C}",

year = "2002",

month = apr,

doi = "10.1105/tpc.010480",

language = "English",

volume = "14",

pages = "857--867",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

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TY - JOUR

T1 - Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase

AU - Vaistij, Fabián E

AU - Jones, Louise

AU - Baulcombe, David C

PY - 2002/4

Y1 - 2002/4

N2 - RNA silencing is a sequence-specific RNA degradation process that follows the recognition of double-stranded RNA. Here, we show that virus vectors carrying parts of a green fluorescent protein (GFP) transgene targeted RNA silencing in Nicotiana benthamiana and Arabidopsis against the entire GFP RNA. These results indicate that there was spreading of RNA targeting from the initiator region into the adjacent 5'and 3' regions of the target gene. Spreading was accompanied by methylation of the corresponding GFP DNA. It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2. These findings indicate that SDE1/SGS2 produces doublestranded RNA using the target RNA as a template. RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2.

AB - RNA silencing is a sequence-specific RNA degradation process that follows the recognition of double-stranded RNA. Here, we show that virus vectors carrying parts of a green fluorescent protein (GFP) transgene targeted RNA silencing in Nicotiana benthamiana and Arabidopsis against the entire GFP RNA. These results indicate that there was spreading of RNA targeting from the initiator region into the adjacent 5'and 3' regions of the target gene. Spreading was accompanied by methylation of the corresponding GFP DNA. It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2. These findings indicate that SDE1/SGS2 produces doublestranded RNA using the target RNA as a template. RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - DNA Methylation

KW - Gene Silencing

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Oxidoreductases

KW - Plant Proteins

KW - Plants, Genetically Modified

KW - RNA Replicase

KW - RNA, Plant

KW - RNA-Induced Silencing Complex

KW - Ribonucleoproteins

KW - Ribulose-Bisphosphate Carboxylase

KW - Tobacco

KW - Transcription, Genetic

U2 - 10.1105/tpc.010480

DO - 10.1105/tpc.010480

M3 - Article

C2 - 11971140

SN - 1040-4651

VL - 14

SP - 857

EP - 867

JO - The Plant Cell

JF - The Plant Cell

IS - 4

ER -