STABILITY OF MUTANT ACTINS

D R Drummond; E S Hennessey; J C Sparrow

STABILITY OF MUTANT ACTINS

D R Drummond, E S Hennessey, J C Sparrow

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

Original language	English
Pages (from-to)	301-303
Number of pages	3
Journal	Biochemical journal
Volume	274
Publication status	Published - 15 Feb 1991

Keywords

INTRACELLULAR PROTEIN DEGRADATION
BACTERIAL-CELLS
ELECTROPHORESIS
TURNOVER
UREA

Cite this

@article{cb09b2dde02a453a8c13c8a152e935dc,

title = "STABILITY OF MUTANT ACTINS",

abstract = "Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.",

keywords = "INTRACELLULAR PROTEIN DEGRADATION, BACTERIAL-CELLS, ELECTROPHORESIS, TURNOVER, UREA",

author = "Drummond, {D R} and Hennessey, {E S} and Sparrow, {J C}",

year = "1991",

month = feb,

day = "15",

language = "English",

volume = "274",

pages = "301--303",

journal = "Biochemical journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

}

TY - JOUR

T1 - STABILITY OF MUTANT ACTINS

AU - Drummond, D R

AU - Hennessey, E S

AU - Sparrow, J C

PY - 1991/2/15

Y1 - 1991/2/15

N2 - Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

AB - Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

KW - INTRACELLULAR PROTEIN DEGRADATION

KW - BACTERIAL-CELLS

KW - ELECTROPHORESIS

KW - TURNOVER

KW - UREA

M3 - Article

SN - 0264-6021

VL - 274

SP - 301

EP - 303

JO - Biochemical journal

JF - Biochemical journal

ER -