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Stoichiometry and architecture of active DNA replication machinery in Escherichia coli

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Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. / Reyes-Lamothe, Rodrigo; Sherratt, David J; Leake, Mark C.

In: Science, Vol. 328, No. 5977, 23.04.2010, p. 498-501.

Research output: Contribution to journalArticle

Harvard

Reyes-Lamothe, R, Sherratt, DJ & Leake, MC 2010, 'Stoichiometry and architecture of active DNA replication machinery in Escherichia coli', Science, vol. 328, no. 5977, pp. 498-501. https://doi.org/10.1126/science.1185757

APA

Reyes-Lamothe, R., Sherratt, D. J., & Leake, M. C. (2010). Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. Science, 328(5977), 498-501. https://doi.org/10.1126/science.1185757

Vancouver

Reyes-Lamothe R, Sherratt DJ, Leake MC. Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. Science. 2010 Apr 23;328(5977):498-501. https://doi.org/10.1126/science.1185757

Author

Reyes-Lamothe, Rodrigo ; Sherratt, David J ; Leake, Mark C. / Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. In: Science. 2010 ; Vol. 328, No. 5977. pp. 498-501.

Bibtex - Download

@article{d106e08469db4801bb0b03073002757c,
title = "Stoichiometry and architecture of active DNA replication machinery in Escherichia coli",
abstract = "The multiprotein replisome complex that replicates DNA has been extensively characterized in vitro, but its composition and architecture in vivo is unknown. Using millisecond single-molecule fluorescence microscopy in living cells expressing fluorescent derivatives of replisome components, we have examined replisome stoichiometry and architecture. Active Escherichia coli replisomes contain three molecules of the replicative polymerase, rather than the historically accepted two. These are associated with three molecules of tau, a clamp loader component that trimerizes polymerase. Only two of the three sliding clamps are always associated with the core replisome. Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines.",
author = "Rodrigo Reyes-Lamothe and Sherratt, {David J} and Leake, {Mark C}",
year = "2010",
month = "4",
day = "23",
doi = "10.1126/science.1185757",
language = "English",
volume = "328",
pages = "498--501",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5977",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Stoichiometry and architecture of active DNA replication machinery in Escherichia coli

AU - Reyes-Lamothe, Rodrigo

AU - Sherratt, David J

AU - Leake, Mark C

PY - 2010/4/23

Y1 - 2010/4/23

N2 - The multiprotein replisome complex that replicates DNA has been extensively characterized in vitro, but its composition and architecture in vivo is unknown. Using millisecond single-molecule fluorescence microscopy in living cells expressing fluorescent derivatives of replisome components, we have examined replisome stoichiometry and architecture. Active Escherichia coli replisomes contain three molecules of the replicative polymerase, rather than the historically accepted two. These are associated with three molecules of tau, a clamp loader component that trimerizes polymerase. Only two of the three sliding clamps are always associated with the core replisome. Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines.

AB - The multiprotein replisome complex that replicates DNA has been extensively characterized in vitro, but its composition and architecture in vivo is unknown. Using millisecond single-molecule fluorescence microscopy in living cells expressing fluorescent derivatives of replisome components, we have examined replisome stoichiometry and architecture. Active Escherichia coli replisomes contain three molecules of the replicative polymerase, rather than the historically accepted two. These are associated with three molecules of tau, a clamp loader component that trimerizes polymerase. Only two of the three sliding clamps are always associated with the core replisome. Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines.

UR - http://www.scopus.com/inward/record.url?scp=77951537332&partnerID=8YFLogxK

U2 - 10.1126/science.1185757

DO - 10.1126/science.1185757

M3 - Article

VL - 328

SP - 498

EP - 501

JO - Science

T2 - Science

JF - Science

SN - 0036-8075

IS - 5977

ER -