By the same authors

From the same journal

From the same journal

Structural basis for DNA strand separation by a hexameric replicative helicase

Research output: Contribution to journalArticle

Standard

Structural basis for DNA strand separation by a hexameric replicative helicase. / Chaban, Yuriy; Stead, Jonathan A; Ryzhenkova, Ksenia; Whelan, Fiona; Lamber, Ekaterina P; Antson, Alfred; Sanders, Cyril M; Orlova, Elena V.

In: Nucleic Acids Research, 03.08.2015.

Research output: Contribution to journalArticle

Harvard

Chaban, Y, Stead, JA, Ryzhenkova, K, Whelan, F, Lamber, EP, Antson, A, Sanders, CM & Orlova, EV 2015, 'Structural basis for DNA strand separation by a hexameric replicative helicase', Nucleic Acids Research. https://doi.org/10.1093/nar/gkv778

APA

Chaban, Y., Stead, J. A., Ryzhenkova, K., Whelan, F., Lamber, E. P., Antson, A., ... Orlova, E. V. (2015). Structural basis for DNA strand separation by a hexameric replicative helicase. Nucleic Acids Research. https://doi.org/10.1093/nar/gkv778

Vancouver

Chaban Y, Stead JA, Ryzhenkova K, Whelan F, Lamber EP, Antson A et al. Structural basis for DNA strand separation by a hexameric replicative helicase. Nucleic Acids Research. 2015 Aug 3. https://doi.org/10.1093/nar/gkv778

Author

Chaban, Yuriy ; Stead, Jonathan A ; Ryzhenkova, Ksenia ; Whelan, Fiona ; Lamber, Ekaterina P ; Antson, Alfred ; Sanders, Cyril M ; Orlova, Elena V. / Structural basis for DNA strand separation by a hexameric replicative helicase. In: Nucleic Acids Research. 2015.

Bibtex - Download

@article{82705138eedd4f70b84fececdf6ed38b,
title = "Structural basis for DNA strand separation by a hexameric replicative helicase",
abstract = "Hexameric helicases are processive DNA unwinding machines but how they engage with a replication fork during unwinding is unknown. Using electron microscopy and single particle analysis we determined structures of the intact hexameric helicase E1 from papillomavirus and two complexes of E1 bound to a DNA replication fork end-labelled with protein tags. By labelling a DNA replication fork with streptavidin (dsDNA end) and Fab (5' ssDNA) we located the positions of these labels on the helicase surface, showing that at least 10 bp of dsDNA enter the E1 helicase via a side tunnel. In the currently accepted 'steric exclusion' model for dsDNA unwinding, the active 3' ssDNA strand is pulled through a central tunnel of the helicase motor domain as the dsDNA strands are wedged apart outside the protein assembly. Our structural observations together with nuclease footprinting assays indicate otherwise: strand separation is taking place inside E1 in a chamber above the helicase domain and the 5' passive ssDNA strands exits the assembly through a separate tunnel opposite to the dsDNA entry point. Our data therefore suggest an alternative to the current general model for DNA unwinding by hexameric helicases.",
author = "Yuriy Chaban and Stead, {Jonathan A} and Ksenia Ryzhenkova and Fiona Whelan and Lamber, {Ekaterina P} and Alfred Antson and Sanders, {Cyril M} and Orlova, {Elena V}",
note = "{\circledC} The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2015",
month = "8",
day = "3",
doi = "10.1093/nar/gkv778",
language = "English",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Structural basis for DNA strand separation by a hexameric replicative helicase

AU - Chaban, Yuriy

AU - Stead, Jonathan A

AU - Ryzhenkova, Ksenia

AU - Whelan, Fiona

AU - Lamber, Ekaterina P

AU - Antson, Alfred

AU - Sanders, Cyril M

AU - Orlova, Elena V

N1 - © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2015/8/3

Y1 - 2015/8/3

N2 - Hexameric helicases are processive DNA unwinding machines but how they engage with a replication fork during unwinding is unknown. Using electron microscopy and single particle analysis we determined structures of the intact hexameric helicase E1 from papillomavirus and two complexes of E1 bound to a DNA replication fork end-labelled with protein tags. By labelling a DNA replication fork with streptavidin (dsDNA end) and Fab (5' ssDNA) we located the positions of these labels on the helicase surface, showing that at least 10 bp of dsDNA enter the E1 helicase via a side tunnel. In the currently accepted 'steric exclusion' model for dsDNA unwinding, the active 3' ssDNA strand is pulled through a central tunnel of the helicase motor domain as the dsDNA strands are wedged apart outside the protein assembly. Our structural observations together with nuclease footprinting assays indicate otherwise: strand separation is taking place inside E1 in a chamber above the helicase domain and the 5' passive ssDNA strands exits the assembly through a separate tunnel opposite to the dsDNA entry point. Our data therefore suggest an alternative to the current general model for DNA unwinding by hexameric helicases.

AB - Hexameric helicases are processive DNA unwinding machines but how they engage with a replication fork during unwinding is unknown. Using electron microscopy and single particle analysis we determined structures of the intact hexameric helicase E1 from papillomavirus and two complexes of E1 bound to a DNA replication fork end-labelled with protein tags. By labelling a DNA replication fork with streptavidin (dsDNA end) and Fab (5' ssDNA) we located the positions of these labels on the helicase surface, showing that at least 10 bp of dsDNA enter the E1 helicase via a side tunnel. In the currently accepted 'steric exclusion' model for dsDNA unwinding, the active 3' ssDNA strand is pulled through a central tunnel of the helicase motor domain as the dsDNA strands are wedged apart outside the protein assembly. Our structural observations together with nuclease footprinting assays indicate otherwise: strand separation is taking place inside E1 in a chamber above the helicase domain and the 5' passive ssDNA strands exits the assembly through a separate tunnel opposite to the dsDNA entry point. Our data therefore suggest an alternative to the current general model for DNA unwinding by hexameric helicases.

U2 - 10.1093/nar/gkv778

DO - 10.1093/nar/gkv778

M3 - Article

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

ER -