Abstract
Anionic glycosarninoglycan mono- and disaccharides (IVA, IH and IS) were subjected to very high-energy collisions (50-150 keV ion kinetic energy prior to collision) with Neon gas in an accelerator mass spectrometer, to explore the possibility of using this method to structurally characterize anionic sugars. Experiments were also conducted for the Na+center dot IH2- and Na+center dot IS3- sodiated complexes. This high-energy ion collision technique is applied here to sugars for the first time. Low-energy collision-induced dissociation (CID) measurements obtained using resonance excitation in a quadrupole ion-trap are presented for comparison. The high-energy measurements produce a rich variety of fragment ions, illustrating the general utility of the technique for providing detailed information for structurally characterizing sugar ions. We discuss the observed fragmentation patterns with reference to the known fragmentation behavior of small gas-phase monoanions, multiply charged anions and cation-dianion complexes. (C) 2009 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 70-77 |
| Number of pages | 8 |
| Journal | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY |
| Volume | 285 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1 Aug 2009 |
Keywords
- Glycosaminoglycan
- Multiply charged anions
- Sulphated sugars
- Collision-induced dissociation
- High-energy mass spectrometry
- ELECTRON DETACHMENT DISSOCIATION
- ZEKE PHOTOELECTRON-SPECTROSCOPY
- TANDEM MASS-SPECTROMETRY
- GAS-PHASE
- ION-TRAP
- HEPARAN-SULFATE
- METAL-COMPLEX
- ANIONS
- IONIZATION
- CAPTURE