STRUCTURE AND FUNCTION OF ENDOGLUCANASE V

G J  DAVIES; G G  DODSON; R E  HUBBARD; S P  TOLLEY; Z  DAUTER; K S  WILSON; C  HJORT; J M  MIKKELSEN; G  RASMUSSEN; M  SCHULEIN

doi:10.1038/365362a0

STRUCTURE AND FUNCTION OF ENDOGLUCANASE V

G J DAVIES, G G DODSON, R E HUBBARD, S P TOLLEY, Z DAUTER, K S WILSON, C HJORT, J M MIKKELSEN, G RASMUSSEN, M SCHULEIN

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

CELLULOSE is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial1 and fungal2 organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families3. The three-dimensional structures of members of two of these families are known4,5. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein6. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.

Original language	English
Pages (from-to)	362-364
Number of pages	3
Journal	Nature
Volume	365
Issue number	6444
DOIs	https://doi.org/10.1038/365362a0
Publication status	Published - 23 Sept 1993

Keywords

3-DIMENSIONAL STRUCTURE
PROTEIN STRUCTURES
CELLULASES
REFINEMENT

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10.1038/365362a0

http://www.nature.com/nature/journal/v365/n6444/abs/365362a0.html

Cite this

@article{fca705668828416c8149ba514896b112,

title = "STRUCTURE AND FUNCTION OF ENDOGLUCANASE V",

abstract = "CELLULOSE is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial1 and fungal2 organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families3. The three-dimensional structures of members of two of these families are known4,5. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein6. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.",

keywords = "3-DIMENSIONAL STRUCTURE, PROTEIN STRUCTURES, CELLULASES, REFINEMENT",

author = "DAVIES, {G J} and DODSON, {G G} and HUBBARD, {R E} and TOLLEY, {S P} and Z DAUTER and WILSON, {K S} and C HJORT and MIKKELSEN, {J M} and G RASMUSSEN and M SCHULEIN",

year = "1993",

month = sep,

day = "23",

doi = "10.1038/365362a0",

language = "English",

volume = "365",

pages = "362--364",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "6444",

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TY - JOUR

T1 - STRUCTURE AND FUNCTION OF ENDOGLUCANASE V

AU - DAVIES, G J

AU - DODSON, G G

AU - HUBBARD, R E

AU - TOLLEY, S P

AU - DAUTER, Z

AU - WILSON, K S

AU - HJORT, C

AU - MIKKELSEN, J M

AU - RASMUSSEN, G

AU - SCHULEIN, M

PY - 1993/9/23

Y1 - 1993/9/23

N2 - CELLULOSE is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial1 and fungal2 organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families3. The three-dimensional structures of members of two of these families are known4,5. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein6. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.

AB - CELLULOSE is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial1 and fungal2 organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families3. The three-dimensional structures of members of two of these families are known4,5. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein6. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.

KW - 3-DIMENSIONAL STRUCTURE

KW - PROTEIN STRUCTURES

KW - CELLULASES

KW - REFINEMENT

U2 - 10.1038/365362a0

DO - 10.1038/365362a0

M3 - Article

SN - 0028-0836

VL - 365

SP - 362

EP - 364

JO - Nature

JF - Nature

IS - 6444

ER -