Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis

R  Grenha; V M  Levdikov; M J  Fogg; E V  Blagova; J A  Brannigan; A.J. Wilkinson; K S  Wilson

doi:10.1107/S174430910501095X

Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis

R Grenha, V M Levdikov, M J Fogg, E V Blagova, J A Brannigan, A.J. Wilkinson, K S Wilson

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since these are recognized as potential targets in antibacterial therapy. Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway. The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis has been solved by molecular replacement at 2.24 Å resolution and refined to an R factor of 18.4%. This is the first report of a DeoD structure from a Gram-positive bacterium.

Original language	English
Pages (from-to)	459-462
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	5
DOIs	https://doi.org/10.1107/S174430910501095X
Publication status	Published - May 2005

Bibliographical note

Keywords

CRYSTAL-STRUCTURE
ESCHERICHIA-COLI
SEQUENCE
DISTINCT
MODEL

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http://www.iucr.org/cgi-bin/paper?gx5050

Cite this

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title = "Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis",

abstract = "Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since these are recognized as potential targets in antibacterial therapy. Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway. The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis has been solved by molecular replacement at 2.24 {\AA} resolution and refined to an R factor of 18.4%. This is the first report of a DeoD structure from a Gram-positive bacterium.",

keywords = "CRYSTAL-STRUCTURE, ESCHERICHIA-COLI, SEQUENCE, DISTINCT, MODEL",

author = "R Grenha and Levdikov, {V M} and Fogg, {M J} and Blagova, {E V} and Brannigan, {J A} and A.J. Wilkinson and Wilson, {K S}",

note = "Copyright {\textcopyright} 2005 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?gx5050",

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AU - Grenha, R

AU - Levdikov, V M

AU - Fogg, M J

AU - Blagova, E V

AU - Brannigan, J A

AU - Wilkinson, A.J.

AU - Wilson, K S

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KW - CRYSTAL-STRUCTURE

KW - ESCHERICHIA-COLI

KW - SEQUENCE

KW - DISTINCT

KW - MODEL

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