By the same authors

From the same journal

Structure of the catalytic core module of the Chaetomium thermophilum family GH6 cellobiohydrolase Cel6A

Research output: Contribution to journalArticlepeer-review

Published copy (DOI)

Author(s)

Department/unit(s)

Publication details

JournalActa Crystallographica Section D: Biological Crystallography
DatePublished - Aug 2012
Issue number8
Volume68
Number of pages8
Pages (from-to)875-882
Original languageEnglish

Abstract

Cellulases, including cellobiohydrolases and endoglucanases, are important enzymes involved in the breakdown of the polysaccharide cellulose. These catalysts have found widescale industrial applications, particularly in the paper and textile industries, and are now finding use in 'second-generation' conversion of biomass to biofuels. Despite this considerable biotechnological application, and undoubted future potential, uncertainty remains as to the exact reaction mechanism of the inverting cellulases found in the GH6 family of carbohydrate-active enzymes. In order to gain additional understanding as to how these societally beneficial biocatalysts function, the crystal structure of a GH6 cellobiohydrolase from Chaetomium thermophilum, CtCel6A, has been solved. This structure reveals a distorted alpha/beta-barrel fold comprising a buried tunnel-like active site quite typical of Cel6A enzymes. Analysis of an enzyme-product complex (cellobiose in the -3 and -2 subsites and cellotetraose in subsites +1 to +4) supports the hypothesis that this group of enzymes act via an atypical single-displacement mechanism. Of particular note in this analysis is an active-centre metal ion, Li+, the position of which matches the position of the positively charged anomeric carbon of the oxocarbenium-ion-like transition state.

    Research areas

  • CRYSTALLINE CELLULOSE, CARBOHYDRATE-BINDING MODULES, MOLECULAR-GRAPHICS, HUMICOLA-INSOLENS, TRICHODERMA-REESEI, THERMOSTABLE ENZYMES, HYDROLYSIS, TRANSITION-STATE ANALOGS, CELLULASES, ACTIVE-SITE

Projects

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations