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Structure of the large terminase from a hyperthermophilic virus reveals a unique mechanism for oligomerization and ATP hydrolysis

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JournalNucleic Acids Research
DateAccepted/In press - 13 Oct 2017
DatePublished (current) - 24 Oct 2017
Issue number22
Volume45
Number of pages14
Pages (from-to)13029-13042
Original languageEnglish

Abstract

The crystal structure of the large terminase from the Geobacillus stearothermophilus bacteriophage D6E shows a unique relative orientation of the N-terminal adenosine triphosphatase (ATPase) and C-terminal nuclease domains. This monomeric ‘initiation’ state with the two domains ‘locked’ together is stabilized via a conserved C-terminal arm, which may interact with the portal protein during motor assembly, as predicted for several bacteriophages. Further work supports the formation of an active oligomeric state: (i) AUC data demonstrate the presence of oligomers; (ii) mutational analysis reveals a trans-arginine finger, R158, indispensable for ATP hydrolysis; (iii) the location of this arginine is conserved with the HerA/FtsK ATPase superfamily; (iv) a molecular docking model of the pentamer is compatible with the location of the identified arginine finger. However, this pentameric model is structurally incompatible with the monomeric ‘initiation’ state and is supported by the observed increase in kcat of ATP hydrolysis, from 7.8 ± 0.1 min−1 to 457.7 ± 9.2 min−1 upon removal of the C-terminal nuclease domain. Taken together, these structural, biophysical and biochemical data suggest a model where transition from the ‘initiation’ state into a catalytically competent pentameric state, is accompanied by substantial domain rearrangements, triggered by the removal of the C-terminal arm from the ATPase active site.

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© The Author(s) 2017.

    Research areas

  • Adenosine Triphosphatases/chemistry, Adenosine Triphosphate/metabolism, Amino Acid Sequence, Bacteriophages/enzymology, Crystallography, X-Ray, Endodeoxyribonucleases/chemistry, Geobacillus stearothermophilus/virology, Hot Temperature, Hydrolysis, Models, Molecular, Mutation, Protein Conformation, Protein Multimerization, Sequence Homology, Amino Acid, Viral Proteins/chemistry

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