Activities per year
Abstract
Sporulation in Bacillus subtilis begins with an asymmetric cell division producing two genetically identical cells with different fates. SpoIIE is a membrane protein that localizes to the polar cell division sites where it causes FtsZ to relocate from mid-cell to form polar Z-rings. Following polar septation, SpoIIE establishes compartment-specific gene expression in the smaller forespore cell by dephosphorylating the anti-sigma factor antagonist SpoIIAA, leading to the release of the RNA polymerase sigma factor sigma(F) from an inhibitory complex with the anti-sigma factor SpoIIAB. SpoIIE therefore couples morphological development to differential gene expression. Here, we determined the crystal structure of the phosphatase domain of SpoIIE to 2.6 angstrom spacing, revealing a domain-swapped dimer. SEC-MALLS (size-exclusion chromatography with multi-angle laser light scattering) analysis however suggested a monomer as the principal form in solution. A model for the monomer was derived from the domain-swapped dimer in which 2 five-stranded beta-sheets are packed against one another and flanked by alpha-helices in an alpha beta beta alpha arrangement reminiscent of other PP2C-type phosphatases. A flap region that controls access of substrates to the active site in other PP2C phosphatases is diminished in SpoIIE, and this observation correlates with the presence of a single manganese ion in the active site of SpoIIE in contrast to the two or three metal ions present in other PP2C enzymes. Mapping of a catalogue of mutational data onto the structure shows a clustering of sites whose point mutation interferes with the proper coupling of asymmetric septum formation to sigma factor activation and identifies a surface involved in intramolecular signaling. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 343-358 |
Number of pages | 16 |
Journal | Journal of Molecular Biology |
Volume | 415 |
Issue number | 2 |
DOIs | |
Publication status | Published - 13 Jan 2012 |
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Seminar Marburg, Germany
Wilkinson, A. J. (Invited speaker)
2 Mar 2019Activity: Talk or presentation › Invited talk
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Le Centre de Biochemie structurale fete ses 25 ans
Wilkinson, A. J. (Invited speaker)
24 May 2018 → 25 May 2018Activity: Participating in or organising an event › Conference
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BACELL 2014
Wilkinson, A. J. (Invited speaker)
29 Apr 2014Activity: Participating in or organising an event › Conference participation
Projects
- 1 Finished
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BaSysBio
Wilkinson, A. J. (Principal investigator)
1/11/06 → 30/04/11
Project: Research project (funded) › Research